Extracting method and detecting method for hemp compounds in hair

A technology of cannabinoid compounds and extraction methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not meeting the detection requirements, low THC content, increasing sample collection volume, etc., and achieves good accuracy and extraction Fast, easy-to-use effects
CN108802240AInactive Publication Date: 2018-11-13广州正孚检测技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州正孚检测技术有限公司
Publication Date
2018-11-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an extracting method and a detecting method for hemp compounds in hair. The extracting method includes: (1) cleaning the hair, and grinding the hair with liquid nitrogen to obtain hair powder; (2) adding the ground hair powder into a sodium hydroxide solution, ultrasonic treating, centrifuging to obtain supernatant, and adding phosphate buffer to buffer so as to obtain treated hair liquid; (3) pouring the treated hair liquid into a balanced solid-phase extraction column, successively washing off impurities with an aqueous acetic acid solution and an aqueous methyl alcohol solution, eluting with a n-hexane-acetic acid solution, and collecting eluant to obtain the hemp compounds. The collected eluant is detected by an LC-MS / MS (liquid chromatogram-mass spectrum / massspectrum) method, rare tetrahydrocannabinol and tetrahydrocannabinol metabolites content in the hair can be detected effectively. The extracting method is simple to operate, mild in extracting conditions and high in extracting speed and releasing efficiency, and the detecting method is simple to operate and high in detection sensitivity.
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Description

Technical field

[0001] The invention relates to biomedicine detection technology, in particular to a method for extracting and detecting cannabis compounds in hair. Background technique

[0002] In recent years, the hair samples have more comprehensive information about drug abuse because of their responses. In order to confirm whether the examinee is taking drugs and the degree of drug ingestion, such samples are often used as evidence for judicial identification and forensic science, and are also widely used in clinical toxicology. , Doping control and other fields.

[0003] The composition of hair is complex, and there are a large number of interfering substances of organic components. In order to detect the THC in the hair, a specific pretreatment method must be established to separate and extract the target substance. Because tetrahydrocannabinol and tetrahydrocannabinol acid are acidic compounds, they are more difficult to enter into black hair than other alkaloid drugs, so ...

Claims

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