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Method for preparing Enterococcus faecalis fermented mixed feed for birds

A technology of Enterococcus faecalis and mixed feed, which is applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of spread and human harm, and achieve the effect of simple production process, growth promotion, and efficient weight gain.

Inactive Publication Date: 2009-01-28
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For the feed used in poultry breeding, on the one hand, it is necessary to consider the nutrients in the feed to improve the cost performance of the feed, and on the other hand, it is also necessary to consider whether the feed itself can improve the resistance of the poultry to pathogenic microorganisms. Ordinary feeds are often added with "three elements". That is: antibiotics, hormones and vitamins, but the abuse of "three elements" will affect the quality of poultry meat and eggs as human food, causing potential harm to humans. Therefore, there is an urgent need for a nutritional labeling that can improve roughage , so that the plant fiber can be converted into a feed that is easily absorbed by small molecules of poultry and can improve the resistance of poultry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:

[0022] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under aerobic conditions at 36°C for 18 hours to become first-class seeds;

[0023] (2) Inoculate the primary seed solution in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under aerobic conditions at 36° C. for 18 hours to become secondary seeds;

[0024] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt a timing fermentation method to cultivate for 18 hours under aerobic conditions at 36° C. to become tertiary seeds;

[0025] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:1:1, and then uniformly mix them with the third-grade seed liquid in a mass ratio of 100:0.6, and then ferment and cultivate fo...

Embodiment 2

[0028] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:

[0029] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under aerobic conditions at 45°C for 4 hours to become first-class seeds;

[0030] (2) Inoculate the primary seed solution in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under aerobic conditions at 45° C. for 4 hours to become secondary seeds;

[0031] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt continuous fed-batch fermentation at 45°C for 8 hours to become the tertiary seed ;

[0032] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:5:5, and then uniformly mix them with the third-grade seed liquid in a mass ratio of 100:0.2, and then ferment and cultivate for 36 hours to obtain No. - ferme...

Embodiment 3

[0035] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:

[0036] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under facultative conditions at 30°C for 24 hours to become first-class seeds;

[0037] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under 30° C. facultative conditions for 24 hours to become secondary seeds;

[0038] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt a timing fermentation method to cultivate for 36 hours at 30° C. or facultative conditions to become a tertiary seed;

[0039] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:5:1, and then uniformly mix with the third-grade seed liquid in a mass ratio of 100:1, and then ferment and cultivate for ...

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Abstract

The invention discloses a method for preparing a poultry enterococcus faecalis mixed feed. The preparation method comprises steps that (1) MRS culture medium is used for culturing FQ15 enterococcus faecalis to become a first level seed; (2) enlargement cultivation is carried out in a starter can; MRS culture medium is adopted as a culture medium to culture the first level seed so and the first level seed becomes a second level seed; (3)fermentation cultivation is carried out in a fermentor; MRS culture medium is adopted as a fermentation culture medium to culture the second level seed and the second level seed becomes a third level seed; (4)rice bran, bran, and cut maize are mixed together and well blended with the third level seed liquid, the mixture are fermented and cultured to be prepared into a first fermentation feed; (5)the first fermentation feed, bean cake, bran, fish meal, bone meal, shell power and salt are blended into the poultry enterococcus faecalis mixed feed. The method of the invention can improve the nutritional content of coarse fodder and ensures that plant fiber is transformed to small molecules materials which are easily absorbed by poultry. When the mixed feed is applied to poultry, antibiotics and hormones are not used, the mixed feed can promote the growth of poultry.

Description

technical field [0001] The invention relates to a preparation method of microbial fermented mixed feed, in particular to a preparation method of poultry Enterococcus faecalis fermented mixed feed. Background technique [0002] For the feed used in poultry breeding, on the one hand, it is necessary to consider the nutrients in the feed to improve the cost performance of the feed, and on the other hand, it is also necessary to consider whether the feed itself can improve the resistance of the poultry to pathogenic microorganisms. Ordinary feeds are often added with "three elements". That is: antibiotics, hormones and vitamins, but the abuse of "three elements" will affect the quality of poultry meat and eggs as human food, causing potential harm to humans. Therefore, there is an urgent need for a nutritional labeling that can improve roughage , so that the plant fiber can be converted into a feed with small molecules that poultry can easily absorb and can improve the resistanc...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/16A23K10/12A23K10/22A23K10/26A23K10/30A23K10/37A23K20/22A23K50/75
CPCY02P60/87
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
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