Method for preparing Enterococcus faecalis fermented mixed feed for birds
A technology of Enterococcus faecalis and mixed feed, which is applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of spread and human harm, and achieve the effect of simple production process, growth promotion, and efficient weight gain.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:
[0022] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under aerobic conditions at 36°C for 18 hours to become first-class seeds;
[0023] (2) Inoculate the primary seed solution in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under aerobic conditions at 36° C. for 18 hours to become secondary seeds;
[0024] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt a timing fermentation method to cultivate for 18 hours under aerobic conditions at 36° C. to become tertiary seeds;
[0025] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:1:1, and then uniformly mix them with the third-grade seed liquid in a mass ratio of 100:0.6, and then ferment and cultivate fo...
Embodiment 2
[0028] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:
[0029] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under aerobic conditions at 45°C for 4 hours to become first-class seeds;
[0030] (2) Inoculate the primary seed solution in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under aerobic conditions at 45° C. for 4 hours to become secondary seeds;
[0031] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt continuous fed-batch fermentation at 45°C for 8 hours to become the tertiary seed ;
[0032] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:5:5, and then uniformly mix them with the third-grade seed liquid in a mass ratio of 100:0.2, and then ferment and cultivate for 36 hours to obtain No. - ferme...
Embodiment 3
[0035] A preparation method for poultry Enterococcus faecalis fermented mixed feed, comprising the steps of:
[0036] (1) Using MRS medium as the seed medium, cultivate FQ15 Enterococcus faecalis (Enterococcus faecalis) CGMCC 2196 under facultative conditions at 30°C for 24 hours to become first-class seeds;
[0037] (2) Inoculate the primary seed liquid in the seed tank for expanded cultivation, adopt MRS medium as the seed medium, and cultivate it under 30° C. facultative conditions for 24 hours to become secondary seeds;
[0038] (3) Inoculate the secondary seed liquid in a fermenter for fermentation and culture, adopt MRS medium as the fermentation medium, and adopt a timing fermentation method to cultivate for 36 hours at 30° C. or facultative conditions to become a tertiary seed;
[0039] (4) Mix rice bran, bran, and crushed corn in a mass ratio of 1:5:1, and then uniformly mix with the third-grade seed liquid in a mass ratio of 100:1, and then ferment and cultivate for ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com