Cosmetic composition containing extracts of pinus koraiensis as active ingredient
A cosmetic composition and active ingredient technology, applied in the direction of medical preparations, cosmetics, cosmetic preparations containing active ingredients, etc., can solve the problems of side effects and insufficient effects, and achieve the promotion of biosynthesis, growth of keratinocytes, The effect of scavenging oxygen free radicals
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[0024] [Reference 1] Preparation of herbal extracts
[0025]Red pine, plum, red bean, papaya, and flax (Kunwha Pharmaceutical Company Ltd.) were collected separately and high-quality herbs were selected, washed to remove impurities and dried in the shade. Then, 100 g of red pine, plum, red bean, papaya and flax were added to 2 L of purified water, and heated and extracted at about 95-100° C. for 18 hours in a reflux device. After the extraction was complete, the extract was then distilled and concentrated to a total weight of about 500 g, then cooled to room temperature. The product is then placed in a filter cloth, separated by pressure and removed from the solids, and filtered to obtain a viscous concentrate. The concentrate was concentrated under vacuum at 80° C. to completely remove water, and dried in vacuum to obtain dry powders of 5.0 g of Korean pine, 10 g of plum, 5.0 g of red bean, 5.0 g of papaya and 5.0 g of flax.
Embodiment 1-15 and comparative example 1
[0027] Each herb extract obtained by water extraction in Reference 1 was mixed as described in Table 1, and used in Examples 1-15 and Comparative Example 1. Here, each herb extract is mixed in the same ratio.
[0028] Table 1
[0029]
experiment Embodiment 1
[0030] [Experimental Example 1]: Efficacy of promoting growth of keratinocytes (MTT assay)
[0031] The MTT method is used to detect the effect of the extracts of Examples 1-15 and Comparative Example 1 on the growth of keratinocytes. The MTT method is briefly described as follows:
[0032] First, keratinocytes were treated at a concentration of 5 × 10 3 / 200 μl / well were planted in 96-well plates, and after 24 hours of culture, they were treated with the extracts of Examples 1-15 and Comparative Example 1 at concentrations of 0.0001, 0.001, 0.01 and 0.1% for 48 hours. Then, the culture solution was aspirated and the cells were washed once with PBS (phosphate buffered saline), treated with 100 μl of MTT (brominated methylthiazolyldiphenyl-tetrazolium, Sigma, USA) solution (0.5 mg / ml), in 37°C and 5% CO 2 Cells were cultured for 4 hours. After aspirating the culture solution, the cells were treated with 200 μl of DMSO (dimethyl sulfoxide) solution, stirred on a shaker for 10...
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