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Vascular aging-predicting factor and utilization of the same

A technology for aging prediction and blood vessels, applied in biological testing, measuring devices, chemical instruments and methods, etc.

Inactive Publication Date: 2009-02-11
NAGASAKI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is an application of the fact that glutathione S-transferase recognizes glutathionylated thiols via an enzymatic reaction, but this method involves a number of issues related to sensitivity and specificity

Method used

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  • Vascular aging-predicting factor and utilization of the same
  • Vascular aging-predicting factor and utilization of the same
  • Vascular aging-predicting factor and utilization of the same

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Experimental program
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Embodiment

[0079] Apolipoprotein B100 protein carbon-terminal 10 amino acid residues (VPSCKLDFRE: SEQ ID NO: 2) containing reactive sulfhydryl groups were synthesized (SIGMA). Glutathione (100 mM) was added to the synthesized peptide solution, followed by incubation at 37° C. for 15 minutes in the presence of hydrogen peroxide (0.5 mM) to glutathionylate the thiol groups. Glutathionylation was eliminated by reduction treatment with a reducing agent, so it was confirmed that the sulfhydryl group was in a state of reversible -S-SG (glutathionylation). Preparation of specific antibodies against peptides having glutathionylated sulfhydryl groups in rabbits (see Susanne Mohr et al., The Journal of Biological Chemistry, vol.274(14), pp. 9427-9430 (1999); Hjelle O.P. et al., Eur. J. Neurosci., vol. 6(5), pp. 793-804 (1994) and Cheng G et al., Archives of Biochemistry and Biophysics, vol. 435, pp. 42-49 (2005)).

[0080] "Quantitative determination of glutathionylated apolipoprotein B100 in ser...

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Abstract

It is intended to provide a factor which predicts vascular aging and a method of examining an early lesion caused by vascular aging. More specifically speaking, it is intended to provide: a factor predicting vascular aging which comprises human apolipoprotein B100 having a glutathionated thiol group; a method of examining a lesion caused by vascular aging which comprises measuring human apolipoprotein B100 having a glutathionated thiol group in a blood sample; an antibody capable of specifically recognizing apolipoprotein B100 having a glutathionated thiol group; and a diagnostic or a diagnostic kit for an early lesion caused by vascular aging which contains an antibody capable of recognizing human apolipoprotein B100 having a glutathionated thiol group.

Description

technical field [0001] The present invention relates to predictors of vascular aging and their use. Specifically, the present invention relates to a vascular aging predictor and a detection method thereof using glutathionylation of a specific sulfhydryl group of human apolipoprotein B100 as an index. Background technique [0002] Among metabolic syndrome, which is expected to become more and more popular in the future, diabetes (impaired glucose tolerance), hypertension, hyperlipidemia, obesity, etc., which are underlying diseases of metabolic syndrome, generally function as accelerators of vascular aging. Vascular aging causes myocardial infarction and cerebral infarction through the formation of arteriosclerosis and arterial blockage. However, available objective indicators of vascular aging are those obtained by morphological measurements (eg, ultrasound diagnosis of carotid arteries), and a simple measurement method using patient serum as a sample has not yet been estab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K16/18G01N33/53
CPCG01N2800/32C07K14/775G01N33/6893C07K16/18C07K14/47G01N33/53
Inventor 近藤宇史井原义人浦田芳重后藤信治河野贵明
Owner NAGASAKI UNIVERSITY
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