Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass

A nucleotide sequence and nucleic acid technology, applied in the field of nucleic acid molecules and their corresponding encoded polypeptides, can solve time-consuming and labor-intensive problems

Inactive Publication Date: 2009-02-18
CERES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such a breeding process is both time-consuming and labor-intensive

Method used

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  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass
  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass
  • Nucleotide sequence and corresponding polypeptide for endowing plant with adjusted growth velocity and biomass

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preparation example Construction

[0115] Preparation of soil mixture: Combine 24L SunshineMix #5 soil (Sun Gro Horticulture, Ltd., Bellevue, WA) and 16L Therm-O-Rock vermiculite (Westerterm-O-Rock company ( Therm-O-Rock West, Inc.), Chandler, AZ) were mixed in a cement mixer to produce a 60:40 soil mixture. Add 2 tablespoons of Marathon 1% granules (Hummert, EarthCity, MO), 3 tablespoons to the soil mixture 14-14-14 (Hummert, Earth City, MO) and 1 tablespoon of Peters fertilizer 20-20-20 (JR Peters, Inc., Allentown, PA), said The additives are first added to 3 gallons of water, then added to the soil and mixed thoroughly. The soil mixture is generally used to fill 4-inch diameter flower pots. Then cover the flowerpot with 8-inch square nylon membrane net.

[0116] Planting: Use a 60 mL syringe to aspirate 35 mL of the seed mixture. Add 25 drops to each pot. A transparent propagation dome was placed on the top of the flower pot, and then the pot was placed under 55% shade cloth and subsurface irrigation was perfor...

Embodiment 1

[0226] Example 1-Lead 80 (ME08386); clone 733804 SEQ ID NO.94

[0227] Lead 80 (SEQ ID NO. 94) encodes a 92 amino acid bHLH transcription factor from wheat. Plants modified with this sequence showed:

[0228] ·Enhanced growth, especially on media containing low nitrate;

[0229] · Enhanced photosynthesis on a medium containing low nitrate;

[0230] ·Elongated hypocotyls, narrow leaves and usually flat inflorescences.

[0231] Clone 733804 encodes a bHLH transcription factor, which can increase growth and improve photosynthetic efficiency of plants subjected to nitrogen deficiency stress. Transcription factors usually control the expression of multiple genes in the pathway. For example, clone 733804 can be involved in controlling the expression of several genes in the pathway, such as the carbon flux of the TCA cycle (Yanagisawa et al., 2004). The related Arabidopsis bHLH transcription factor and potential orthologs (60% identity, clone 8607) can also confer a similar low nitrogen...

Embodiment 2

[0256] Example 2-Lead 81 (ME03973) clone 8607 SEQ ID NO. 96

[0257] Leading 81 (SEQ ID NO. ＊＊ ) Encodes a 94 amino acid bHLH transcription factor from Arabidopsis. Plants transformed with this sequence showed:

[0258] ·Enhanced growth, especially on media containing low nitrate;

[0259] · Enhanced photosynthesis on a medium containing low nitrate;

[0260] ·Elongated hypocotyls, narrow leaves and usually flat inflorescences.

[0261] Clone 8607 encodes an Arabidopsis basic helix-loop-helix transcription factor. The clone was placed in a cDNA abnormal expression pipeline to test its utility in improving plant performance under various stress conditions. In heat, drought, and nitrogen deficiency stress experiments, the expression of genes is different, so they can play a role in regulating genes that are important for stress tolerance or adaptability.

[0262] Clone 8607 encodes a bHLH transcription factor, which can increase growth and improve photosynthetic efficiency of plant...

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Abstract

The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of modulated plant size, vegetative growth, organ number, plant architecture, growth rate, seedling vigor and / or biomass in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, organ number, plant architecture, growth rate, seedling vigor and / or biomass that are altered with respect to wild type plants grown under similar conditions.

Description

Technical field [0001] The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides, which can regulate plant growth rate, vegetative growth, organ size, structure, seedling vigor and / or biomass. The present invention further relates to the use of said nucleic acid molecules and polypeptides to enable transgenic plants, plant cells, plant materials or plant seeds to have a regulated growth rate, vegetative growth, and organ number compared to wild-type plants grown under similar conditions , Structure, seedling vigor and / or biomass. Background technique [0002] Specific improvement of plants for agriculture, horticulture, biomass conversion, and other industries (such as the paper industry, plants as factories for the production of proteins or other compounds) can be achieved by molecular technology. For example, by adjusting the size of the whole plant or any of its organs or the number of any of its organs, huge agricultural val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C07K14/415C12N5/04C12N5/10C12Q1/68A01H5/00
CPCC12N15/8261C07K14/415Y02A40/146
Inventor N·亚历山德洛夫V·布罗沃P·马西亚K·A·费尔德曼R·施耐伯格G·纳扎恩
Owner CERES INC
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