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Method for detecting bio bar code for bluetongue virus

A bluetongue virus, biological barcode technology, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve the problems of cross-reaction, low cost, detection sensitivity can only reach ng level, etc. High sensitivity, high specificity, and easy-to-use results

Inactive Publication Date: 2009-03-11
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The AGID test is easy to operate, high in sensitivity, low in cost, and does not require complex experimental equipment. It is one of the earliest widely used bluetongue virus antibody detection methods and is also the method recommended by OIE. If EHDV is cross-reactive
Competitive ELISA (C-ELISA) is the preferred method for serological diagnosis of bluetongue virus recommended by OIE. It is the most sensitive and specific detection technique for bluetongue virus antibody. Meet the requirements of inspection and quarantine
In recent years, the nucleic acid detection of bluetongue virus has been extensively studied, but there is no commercial bluetongue virus nucleic acid detection reagent on the market

Method used

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  • Method for detecting bio bar code for bluetongue virus
  • Method for detecting bio bar code for bluetongue virus
  • Method for detecting bio bar code for bluetongue virus

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Embodiment 1

[0045] Embodiment 1, the biological barcode detection of bluetongue virus

[0046] Carry out qualitative and quantitative detection to bluetongue virus with biological barcode detection method of the present invention, concrete method comprises the following steps:

[0047] 1. Synthesis of oligonucleotide chains

[0048] Design and synthesize the oligonucleotide chain used in the bluetongue virus biological barcode detection method of the present invention, the sequence is as follows:

[0049] Barcoded DNA strands:

[0050] 5'-CGCATTCAGGATTGCATGATTGCCTCGTCTTAACGGTCTCAACTCGTA-3' (SEQ ID No: 1 in the sequence listing)

[0051] Complementary probe NP strand:

[0052] 5'-TACGAGTTGAGACCGTTAAGACGAGGCAACATGCAATCCTGAATGCGA 10 -(CH 2 ) 6 -SH-3' (SEQ ID No: 2 in the sequence listing)

[0053] Capture DNA strand (for chip detection):

[0054] 5'-HS-(CH 2 ) 6 -A 10 -TACGAGTTGAGACCGTTAAGACGA-3'

[0055] NP tag chain (for chip detection):

[0056] 5'-GGCAATCATGCAATCCTGAATGCGA ...

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Abstract

The invention discloses a biological bar code test method of bluetongue virus, taking VP7 protein of the bluetongue virus as a tested object. An MMP probe is coated with an anti-VP7 protein monoclonal antibody, and an NP probe is coated with an anti-VP7 protein polyclonal antibody. A bar code DNA chain carrying out the biological bar code test to the bluetongue virus is provided with an SEQ ID No: 1 nucleotide sequence in a sequence table, while a complementary probe NP chain is provided with an SEQ ID No: 2 nucleotide sequence in the sequence table. The invention has greater practical significance in the field of the diagnosis of the bluetongue and the inspection and quarantine of import and export, thus having wide application prospects.

Description

technical field [0001] The invention relates to a virus detection method in the field of biotechnology, in particular to a biological barcode detection method for bluetongue virus. Background technique [0002] Bluetongue (bluetongue, BT) is caused by bluetongue virus (bluetongue virus, bluetongue virus), a kind of encroachment on ruminant animal-borne infectious disease, by the World Organization for Animal Health (OIE) Listed as a reported disease, it is a key epidemic disease monitored and controlled by animal epidemic prevention and entry-exit inspection and quarantine agencies at home and abroad, and has a very large impact on international animal trade. [0003] With the rapid development of pharmaceutical products, the research and development of animal-derived biological products for human use has become one of the most active and fastest-growing industries, such as human bovine-derived thrombin, bovine-derived coagulation factor VIII, polymerized bovine hemoglobin, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577C12Q1/68
Inventor 章金刚张立营吕茂民马玉媛尹惠琼杨姝李德雪
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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