Use of marsdenia tenacissima new glycoside B in preparing medicine for treating tumor disease and preparation method thereof

A technology for clearing vines and tumors, which is applied in the fields of antitumor drugs, drug combinations, and sugar derivatives preparation, and can solve the problems of complex ingredients, poor quality control, and unfavorable clinical drug safety.

Inactive Publication Date: 2009-04-01
HANGZHOU MINSHENG PHARM CO LTD
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the invention patent "Extraction of Active Components of Xiaoaiping and Preparation of Dosage Forms" (Application No.: 92102027.9), it is believed that the polysaccharides extracted from Aegypti aegypti are its anti-tumor active components, and there are no reports of other active components.
Therefore, it is very unfavorable for quality control and clinical drug safety
[0

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of marsdenia tenacissima new glycoside B in preparing medicine for treating tumor disease and preparation method thereof
  • Use of marsdenia tenacissima new glycoside B in preparing medicine for treating tumor disease and preparation method thereof
  • Use of marsdenia tenacissima new glycoside B in preparing medicine for treating tumor disease and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Preparation of glucoside B

[0072]Take 1000g of the dried cane of S. chinensis, add water to cover the medicinal materials, decoct three times for 1 hour each, combine the decoctions, filter, concentrate the filtrate to 400ml, and let it cool to obtain the extract of S. Dissolve the ointment in 500ml of water, add 500ml of absolute ethanol, shake to precipitate with alcohol, filter, extract the filtrate with chloroform, collect the orange-red clear liquid in the lower layer, and repeatedly extract until the lower layer is colorless. After combining the chloroform extracts, add about 100ml of anhydrous ether , shake the ether to sink, filter, and drain the filtrate. After the extract was dissolved in chloroform, it was separated through a silica gel column, washed with chloroform, and the eluate was collected, and the collected liquid was monitored by thin-layer chromatography. The developing solvent was n-butanol: ethyl acetate: water = 4: 1: 15, and the thin layers we...

Embodiment 2

[0074] Preparation of glucoside B

[0075] Take 1000g of the dried cane of S. chinensis, add water to cover the medicinal materials, decoct three times for 1 hour each, combine the decoctions, filter, concentrate the filtrate to 400ml, and let it cool to obtain the extract of S. Dissolve the ointment in 500ml of water, add 1000ml of absolute ethanol, shake to precipitate with alcohol, filter, extract the filtrate with chloroform, collect the orange-red clear liquid in the lower layer, and repeatedly extract until the lower layer is colorless. After combining the chloroform extracts, add about 200ml of anhydrous ether , shake the ether to sink, filter, and drain the filtrate. After the extract was dissolved in chloroform, it was separated through a silica gel column, rinsed with chloroform, collected the eluent, and monitored by thin-layer chromatography of the collected liquid. The developing solvent was n-butanol: ethyl acetate: water = 4:1:15, and the thin layers were combin...

Embodiment 3

[0077] Preparation of glucoside B

[0078] Take 1000g of the dried cane of S. chinensis, add water to cover the medicinal materials, decoct three times for 1 hour each, combine the decoctions, filter, concentrate the filtrate to 400ml, and let it cool to obtain the extract of S. Dissolve the ointment in 500ml of water, add 750ml of absolute ethanol, shake for alcohol precipitation, filter, and extract the filtrate with chloroform, collect the orange-red clear liquid in the lower layer, and repeatedly extract until the lower layer is colorless. After combining the chloroform extracts, add about 150ml of anhydrous ether , shake the ether to sink, filter, and drain the filtrate. After the extract was dissolved in chloroform, it was separated through a silica gel column, washed with chloroform, and the eluate was collected, and the collected liquid was monitored by thin-layer chromatography. The developing solvent was n-butanol: ethyl acetate: water = 4: 1: 15, and the thin layers...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an application of a compound marsdenia tenacissima glycoside B which is extracted and separated from natural plant marsdenia tenacissima in the preparation of a drug for remedying tumour diseases including various malignant tumour diseases, such as ovary cancer, stomach cancer, liver cancer, leucocythemia, lung cancer, oral epidermoid carcinoma, rectal adenocarcinoma, breast cancer, malignant melanocarcinoma, colon adenocarcinoma, nasopharyngeal carcinoma, prostate cancer, etc. The invention also relates to a preparation technology of the marsdenia tenacissima glycoside B. After using water extraction and alcohol sedimentation, the invention uses an innovative chloroform extraction and aether sedimentation technology by chloroform extraction and also creatively adding aether, thereby reducing the content of impurity in extract obviously, and the marsdenia tenacissima glycoside B with high purity can be obtained just by silica gel column separation at a time. The method has high yield and simple operation, does not require a preparation type precision instrument of high performance liquid phase and other similar instruments and is closer to the requirement of the industrialized production.

Description

technical field [0001] The present invention relates to the application of the compound genus glucoside B extracted and separated from the natural plant Aegyptifolia in the preparation of medicines for treating tumor diseases, and an industrialized extraction and separation method thereof. Background technique [0002] Mersolenia tenocissima (Roxb) is a plant of the genus Milkweed in the family Romoaceae, and its scientific name is Mersolenia tenocissima (Roxb), also known as Tongguansan, Tongguanteng, Dakuteng, Xiamiliteng, etc. It is used as a medicine for treating bronchitis and anti-tumor in Yunnan plant. Since 1970, the 33970 troops of the People's Liberation Army have used the decoction of Wuguteng to treat various malignant tumors and achieved good results. [0003] However, there are few research reports on the anti-tumor active ingredients of Acacia sinensis at home and abroad. In the invention patent "Extraction of Active Components of Xiaoaiping and Preparation ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/704A61K9/10A61K9/107A61K36/185A61P35/00C07H1/08
Inventor 孟昭珂郭殿武吴耀东杜九龄陈楹
Owner HANGZHOU MINSHENG PHARM CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap