Method for eliminating interference of high riglyceride for detecting hemoglobin concentration

A technology of hemoglobin concentration and high triglyceride, applied in the field of hemoglobin concentration measurement, can solve the problems of cumbersome operation, time-consuming, poor accuracy, etc., and achieve the effect of high accuracy, short time and simple operation

Inactive Publication Date: 2009-04-01
WEST CHINA HOSPITAL SICHUAN UNIV
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AI Technical Summary

Problems solved by technology

This method is not only limited by special equipment (high-speed centrifuge), but also cumbersome and time-consuming to operate. During the operation, it was found that the fat particles after high-speed centrifugation clumped into agglomerates and floated on the plasma, making it difficult to completely suck out and measure it accurately. Aspirated volume, thus poor accuracy
Indirect measurement (see Kalache GR, Sartor MM, Hughes WG. The indirect estimation of hemoglobin concentration in whole blood. Pathology. 1991 Apr; 23(2): 115-7.) Mean corpuscular volume (MCV) obtained by standard electronic counting, Calculate the index ratio between MCV and mean hemoglobin content (MCH), and then use the formula to correct HGB. This method is limited by the fact that most hematology analyzers do not have standard electronic counting MCV functions, and it is difficult to be popularized and practical.

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  • Method for eliminating interference of high riglyceride for detecting hemoglobin concentration

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Experimental program
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Effect test

Embodiment 1

[0020] EDTAK 2 (Potassium oxalate tetraacetate) anticoagulated (special concentration for blood routine) normal fresh whole blood (TG<1.83mmol / L, normal reference range of TG is 0.29-1.83mmol / L, no hemolysis, macroscopic observation, erythrocyte sedimentation After the blood plasma is light yellow and transparent), 40 parts are divided into 4 groups, every group has 10 parts, every part of 2ml.

[0021] (1) Complete blood cell count for each part of normal fresh whole blood

[0022] When the XE-2100 blood cell analyzer is ready (the green Ready light is on), invert the test tube containing normal fresh whole blood 8-10 times to mix the blood evenly, then remove the cap of the test tube, and place the normal fresh whole blood in the Under the instrument's sampling needle, confirm that the sampling needle enters the middle of the blood, press the green counting button to aspirate the sample, when the instrument's suction sound stops, remove the test tube, and the concentration ...

Embodiment 2

[0031] Four groups of fat emulsions were prepared with 30% sterile fat emulsion injection and normal plasma (TG<1.83mmol / L, light yellow and transparent), 10 parts in each group (concentrations ranging from 4.76 to 53.28mmol / L) , 2ml per serving.

[0032] (1) Carry out complete blood count (CBC) of each fat emulsion

[0033] When the XE-2100 hematology analyzer is ready (the green Ready light is on), remove the test tube cover, place the fat emulsion under the sampling needle of the instrument, confirm that the sampling needle enters the middle of the fat emulsion, and press the green counting button to suck. In this way, when the suction sound from the instrument stops, remove the test tube, and the detection values ​​of red blood cells (RBC) and hemoglobin (HGB) will be displayed automatically after 1 minute. After data processing, the measured values ​​of erythrocyte and hemoglobin concentrations of each group of fat emulsions were obtained, as shown in Table 2.

[0034] ...

Embodiment 3

[0042] EDTAK 2 120ml of anticoagulated normal fresh whole blood (TG<1.83mmol / L, no hemolysis, observed with naked eyes, plasma after erythrocyte sedimentation is light yellow and transparent) was divided into 60 parts, each 2ml, 10 parts for each group, 6 groups in total.

[0043] (1) Complete blood cell count for each part of normal fresh whole blood

[0044] When the XE-2100 blood cell analyzer is ready (the green Ready light is on), invert the test tube containing normal fresh whole blood 8-10 times to mix the blood evenly, then remove the cap of the test tube, and place the normal fresh whole blood in the Under the instrument's sampling needle, confirm that the sampling needle enters the middle of the blood, press the green counting button to aspirate the sample (130 μl of sample is sucked), and when the suction sound from the instrument stops, remove the test tube, and the hemoglobin concentration will be automatically displayed after 1 minute (HGB) measured value. Afte...

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Abstract

The invention provides an eliminating method of interference of the measurement of hypertriglyceride to the concentration of hemoglobin, comprising the following steps of: (1) carrying out complete blood count to blood containing the hypertriglyceride and obtaining the measuring value, HGB lipemia, of the concentration of the hemoglobin and the hematocrit, HCT lipemia, of the erythrocyte; (2) carrying out low-speed centrifugation to the blood containing the hypertriglyceride in the step (1) and leading the erythrocyte and the blood plasma in the blood to be separated; (3) aspirating the blood plasma separated out in the step (2), carrying out complete blood count to the blood plasma and obtaining the measuring value, HGB lipemia blood plasma, of the concentration of the hemoglobin in the blood plasma; and (4) carrying out correction to the measuring value, HGB lipemia, of the concentration of the hemoglobin obtained in the step (1), obtaining the corrected value of the concentration of the hemoglobin and the corrective formula is as follows: HGB corrected value is equal to HGB lipemia-(HGB lipemia blood plasma minus HGB lipemia blood plasma multiplied by HCT lipemia), in the formula, the HGB lipemia blood plasma multiplied by HCT lipemia is the percentage of the erythrocyte in the blood containing the hypertriglyceride.

Description

technical field [0001] The invention belongs to the field of measuring hemoglobin concentration of blood containing high triglyceride, in particular to a method for eliminating the interference of high triglyceride on the measurement of hemoglobin concentration. Background technique [0002] Experiments have shown that when the triglyceride (TG) content in the blood>4.76mmol / L, it will seriously interfere with the determination of the hemoglobin concentration of the blood routine test. The higher the triglyceride (TG) concentration, the greater the degree of interference, and the result It is a false increase in the measurement results of hemoglobin (HGB) concentration. [0003] In order to accurately obtain the hemoglobin concentration of blood containing high triglycerides, two methods are mainly used at home and abroad, namely high-speed centrifugation and indirect measurement. The operation of the high-speed centrifugation method is: 1. Centrifuge at a speed of 3,500...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 曾素根曾婷婷贾永前江虹周静黄玉霞孙玉明唐书强
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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