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Synthesis of melamine artificial antigen

A technology of melamine and artificial antigen, which is applied in the direction of animal/human protein, material inspection product, albumin peptide, etc., can solve the problem of not being able to meet the requirements of convenience, speed and accuracy, the process of processing and purification is time-consuming and laborious, and the requirements for inspection materials are high, etc. problems, low cost, simple synthesis steps, and easy operation

Inactive Publication Date: 2009-04-08
JIANGNAN UNIV
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Problems solved by technology

At present, there are three methods for the detection of melamine in my country, namely high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), but these three methods require expensive Advanced instruments and equipment and professional technicians have high requirements for inspection materials, and the process of sample pretreatment and purification is time-consuming and laborious, which can no longer meet the requirements of modern detection for convenience, speed and accuracy.
In recent years, studies on melamine immunoassay methods have been carried out abroad, but there is no such report in China. In order to make up for this gap, it is necessary to prepare melamine artificial antigen

Method used

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  • Synthesis of melamine artificial antigen
  • Synthesis of melamine artificial antigen

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Embodiment Construction

[0018] 1. Preparation of Artificial Antigen

[0019] (1) Preparation of liquid A: Weigh 13 mg of hapten into a 25 mL beaker, add 4 mL of pH 7.2 phosphate buffer solution containing 50% formaldehyde in mass concentration, stir magnetically at room temperature until fully dissolved, then add mass 12 mL of glutaraldehyde with a concentration of 25% was reacted at room temperature for 3 hours under magnetic stirring, and this solution was called solution A.

[0020] Phosphate buffer: 10.9g disodium hydrogen phosphate, 3.2g sodium dihydrogen phosphate, 90g sodium chloride, add distilled water to 1000mL, adjust the pH to 7.2, store at room temperature, dilute 10 times when used to be the working solution.

[0021] (2) Preparation of liquid B: Weigh 10 mg of bovine serum albumin and dissolve it in 2 mL of 0.05 mmol / L carbonate buffer, pH 9.6, and store at low temperature. This liquid is B liquid.

[0022] Carbonate buffer: Weigh 1.08g of sodium carbonate, 2.95g of sodium bicarbonat...

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Abstract

The invention discloses a method for synthesizing an artificial antigen of melamine, pertaining to the technical field of biochemical industry. In the method, the melamine is taken as a semiantigen and the existing amino group in the structure thereof is utilized, the semiantigen is combined with bovine serum albumin through glutaral pentanedial, and the artificial antigen of melamine which is melamine-bovine serum albumin is prepared; the products prepared can be applied to the detection of content of melamine in foods and immunity, with advantages of fast detection, convenient operation and low cost. The method has concise and effective synthesis steps, thus being completely capable of being applied to immunity analysis, providing necessary artificial antigen for the future research, and meeting the requirement for the research thereon in China.

Description

technical field [0001] The invention discloses a method for synthesizing an artificial antigen of melamine, which belongs to the technical field of biochemical industry. Background technique [0002] Melamine (Melamine, MM) is referred to as triamine for short, and its scientific name is triaminetriazine, and its aliases are melamine, cyanuramide, and tripolyamide. Used in plastics, coatings, leather and other industries. Melamine is relatively stable under normal circumstances, but it will decompose and release cyanide at high temperature. Therefore, the utensils made of melamine must be marked "do not use in microwave ovens". In addition, melamine can also be used as a flame retardant, adhesive, formaldehyde cleaner, etc. Through hydrolysis, melamine can be converted into ammeline, ammeline, and cyanuric acid. US FDA studies have shown that melamine and calcium form insoluble complexes under the maintenance of urine pH in the body, leading to urinary stones and bladder ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/53
Inventor 胥传来孙凤霞刘丽强彭池方徐丽广谢会玲袁媛尹丽梅
Owner JIANGNAN UNIV
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