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Avirulence gene avrxa5 capable of stimulating rice to generate disease resistance response

A non-toxic gene and gene technology, applied in the gene field in the field of biotechnology, can solve the problem of undiscovered non-toxic gene avrxa5, etc., and achieve the effect of preventing and controlling harm

Inactive Publication Date: 2009-06-10
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After searching the literature of the prior art, it was found that there was no report related to the avirulent gene avrxa5 corresponding to the rice recessive disease resistance gene xa5

Method used

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  • Avirulence gene avrxa5 capable of stimulating rice to generate disease resistance response
  • Avirulence gene avrxa5 capable of stimulating rice to generate disease resistance response
  • Avirulence gene avrxa5 capable of stimulating rice to generate disease resistance response

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Embodiment

[0018] Step 1, cloning of the rice bacterial blight avrxa5 gene

[0019] Xanthomonas oryzae JXOIII strain exhibited non-affinity interaction with rice IRBB5 containing xa5 resistance gene. In this study, JXOIII was used as the target strain, and its genomic DNA was extracted, partially digested by EcoRI and constructed on the vector pUFR034 vector to obtain the JXOIII gene library. figure 1 In this study, using the DNA fragment of the conserved NLS region of the avrBs3 / PthA family gene as a probe, the JXOIII gene library was screened by Southern hybridization, and positive clones containing the avrBs3 / PthA family gene were obtained. figure 1 Among them, B represents the BamHI restriction site, P represents the PstI restriction site, and S represents the SphI restriction site; the mediastinum represents the 102bp repeat unit, which repeats 19.5 times in the avrxa5 gene; LZ represents the leucine zipper domain ; NLS represents the nuclear signal, 1, 2, 3 represent 3 NLSs; AD re...

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Abstract

The invention discloses an avirulence gene avrxa5 for exciting rice to produce disease-resistant response in the technical field of biology. A gene sequence is SEQ ID NO.1,the length of the sequence is 3,717bp, a digestion site on the gene is the same as those of avrBs3 / PthA family genes, and the repeat number of a 102bp repeating unit in the gene is 19.5; and the avirulence gene avrxa5 encodes 1,238 amino acids, the sequence of the amino acid is SEQ ID NO.2, and 34 amino acid repeating units encoded by 102bp are repeated for 19.5 times in protein. The avrxa5 gene can be taken as a gene resource of disease-resistant breeding of resisting bacterial blight of the rice; and after the gene is transferred into an Xanthomonas oryzae pv. oryzae PXO99 strain, the rice containing an xa5 disease-resistant gene can produce the disease-resistant response to make Xanthomonas oryzae pv. oryzae difficult to effectively infect the rice.

Description

technical field [0001] The invention relates to a gene in the field of biotechnology, in particular to an avirulent gene avrxa5 that stimulates rice to produce disease resistance. Background technique [0002] Rice bacterial blight is caused by Xan thomonas oryzae pv. oryzae, and is one of the three major diseases in rice production in my country. It often causes a 5%-25% reduction in rice production, and in severe cases it will lead to a complete failure of the rice harvest. , seriously restricting the safe production of rice in my country. The interaction between bacterial blight and rice conforms to the "gene-to-gene" relationship, that is, if there is an avirulence gene in the pathogen, there is a matching disease resistance gene in rice. The avirulent gene in bacterial blight can stimulate the activity of disease-resistant genes in rice, thereby making rice resistant to disease. So far, the genes that have identified and clarified the functions of avirulent genes in Xa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/195
Inventor 陈功友邹华松邹丽芳赵文祥李玉蓉
Owner SHANGHAI JIAO TONG UNIV
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