Use of Sichuang-Tibet amethystoidin A in preventing and treating acute lymphocyte leukaemia and cervical cancer
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A technology of Sichuan-Tibetan incense tea A, acute lymphocytes, which can be used in the fields of biotechnology and medicine and can solve problems such as drug resistance
Inactive Publication Date: 2009-08-05
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE +1
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However, existing anti-microtubule compounds sti
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Embodiment 1
[0101] Example 1 cell treatment
[0102] The human T-cell leukemia cell line Jurkat and the human B-lymphoma cell line Raji were treated with 10% heat-inactivated fetal bovine serum (FBS, Gibco BRL, Gaithersburg, ML), penicillin (100 IU / ml) and streptomycin (100 μg / ml) in RPMI 1640 medium (Sigma-Aldrich, St Louis, MO), placed at 37°C, containing 5% CO 2 and 95% air incubator. Hela (cervical carcinoma) cells were obtained from the American Standard Biological Collection (ATCC, Manassas, VA) and cultured in Dulbesso containing 10% heat-inactivated fetal calf serum on Petri dishes or on Lab-Tek II slides (Fisher Scientific). 's modified Eagle's medium (DMEM), placed at 37 ° C, containing 5% CO 2 and 95% air incubator. Paclitaxel-resistant Jurkat cells were supplemented with 20 nM paclitaxel (Sigma-Aldrich, St Louis, MO) every other day and maintained for a long time. In the experiment, the cells were 2 × 10 5 Cells / ml were seeded and treated with corresponding concentra...
Embodiment 2
[0103] Example 2 Flow Cytometry Analysis
[0104] For flow cytometry analysis of DNA content, collect 10 6 Cells were rinsed and fixed overnight at -20°C with 75% cold ethanol. Cells were treated with 100 μg / ml RNase A dissolved in Tris-HCl buffer (pH 7.4) and stained with 25 μg / ml propidium iodide (PI, Sigma-Aldrich, St Louis, MO), and samples were analyzed by flow cytometry (FACSCalibur, BD Biosciences, San Jose, CA, USA) CellQuest Pro software (BD Biosciences) was used. 10,000 cells were selected for DNA content analysis.
Embodiment 3
[0105] Example 3 co-immunoprecipitation
[0106] Equal amounts of cell lysates from different treatments were incubated overnight at 4°C with the rabbit polyclonal antibody Cdc20. Protein A Sepharose beads (Santa Cruz, CA) were added for 2 hours and incubated at 4°C. Rabbit IgG (Santa Cruz, CA) was used as a negative control before normal immunization. Immunoprecipitates were collected, rinsed with lysis buffer, and dissolved in 2× sodium dodecyl sulfate (SDS) for western blot analysis.
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Abstract
The invention provides application of Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina in preventing and treating acute lymphoblastic leukemia and cervical cancer. The main application of the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina is that the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina, physiologically or pharmaceutically acceptable salts of the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina, hydrates of the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina or a mixture of the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina, the salts and the hydrates are used to prepare a composition for preventing, controlling or treating diseases or symptoms related to the acute lymphoblastic leukemia and the cervical cancer. The invention also provides a composition containing active ingredients of the Jiasu of the root, leaf or flower of Szechwan-Tibet Rabdosina and other matters having effects of preventing, controlling or treating diseases related to the leukemia and/or diseases or symptoms related to cancers. The composition has the advantages of high safety, easy absorption, good stability, easy market acceptance and the like.
Description
technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to the use of the Sichuan-Tibetan genus A active substance in the prevention, control or treatment of acute lymphoblastic leukemia and cervical cancer. Background technique [0002] The polarized structure of microtubules is assembled by α- and β-tubulin heterodimers in a GTP-dependent manner. Microtubules not only maintain cell shape, but also play an important role in many cellular processes, such as the long-distance transport of intracellular materials or organelles, and the assembly of the mitotic spindle. The motility of microtubules plays an important role in the progression of the cell cycle, and any disturbance of this dynamic process will cause mitotic arrest, often accompanied by apoptosis or mitotic abnormalities. [0003] The successful use of paclitaxel in the clinic has led to more and more research efforts to discover new microtubule-targeting compounds su...
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