Choerospondias extractive, preparation and application of phenol compound contained in choerospondias extractive

A technology of compounds and phenolic acids, which is applied in the field of preparation and application of phenolic acid compounds contained in Suanzi jujube extract, can solve the problem that the anti-hypoxic activity of phenolic acid compounds has not been reported, and the anti-hypoxic active ingredients Issues not yet clarified

Inactive Publication Date: 2009-10-21
INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the hypoxic protective effect of total flavonoids of South Suanzizao fruit has also been documented (Li Zengxi et al.; Protective effect of total flavonoids of Guangzao jujube on animal hypoxia tolerance and acute myocardial ischemia: Chinese Herbal Medicine, 1984, Volume 15, No. 6, pages 25-27), but the anti-hypoxic active ingredient has not been elucidated
The anti-hypoxic activity and application of the extract of Suanzi jujube and the phenolic compounds isolated from it have not been reported so far

Method used

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  • Choerospondias extractive, preparation and application of phenol compound contained in choerospondias extractive
  • Choerospondias extractive, preparation and application of phenol compound contained in choerospondias extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1. Southern jujube extract

[0029] 3.2kg of dry bark of Choerospondias axillaries (Roxb.) Burtt.et Hill. (collected in August 1999 from Mengla area, Yunnan Province) was pulverized and extracted with commercially available medical ethanol at room temperature, each time with 60% (v / v ) in 25 liters of aqueous ethanol soaked for 7 days, extracted 4 times altogether, combined extracts, concentrated and dried under reduced pressure to obtain 750 g of ethanol extract. 750 g of the ethanol extract was suspended in 3 liters of water, extracted successively with chloroform, ethyl acetate, and n-butanol, each solvent was used for 3 liters each, extracted 4 times respectively, the same extracts were combined, concentrated and dried under reduced pressure, and obtained 60 g of the chloroform extract, 310 g of the ethyl acetate extract, 300 g of the n-butanol extract, and 80 g of the aqueous layer residue.

[0030]The medicinal dregs after the above-...

Embodiment 2

[0031] Embodiment 2. The preparation of compound I and IV in the ethyl acetate extract of South Suanzijube

[0032] Column Chromatographic Separation of Ethyl Acetate Extract

[0033] Dissolve 100 g of the ethyl acetate extract obtained in Example 1 with an appropriate amount of methanol, add an appropriate amount of polyamide to absorb, dry, put on a polyamide column (column bed in ethyl acetate 7.0cm×50cm), and use the following Ethyl acetate-methanol gradient elution in different volume ratios, the eluents were collected and combined according to the thin layer detection results, concentrated and dried under reduced pressure to obtain four chromatographic crude components: E-1 (4g, eluted with ethyl acetate fraction), E-2 (35g, fraction eluted with ethyl acetate-methanol 9:1), E-3 (25g, fraction eluted with ethyl acetate-methanol 1:1), and E-4 (15g, fraction eluted with methanol part).

[0034] Component E-2 (35g) was dissolved in an appropriate amount of methanol, and a ...

Embodiment 3

[0041] Example 3. Preparation of compound I to compound VI in the n-butanol extract of Suanzi jujube

[0042] Column Chromatographic Separation of n-Butanol Extract

[0043] Dissolve 300 g of the n-butanol extract of Suanzi jujube obtained in above-mentioned Example 1 with an appropriate amount of methanol, add an appropriate amount of macroporous resin AB-8 for adsorption, dry, and put it on the macroporous resin AB-8 column (column bed in water 8.5cm × 48cm ), with different volume ratios of water-ethanol (100:0 → 0:100) for gradient elution, collect and combine eluents according to the results of thin-layer detection, concentrate and dry under reduced pressure, and obtain two water-washed fractions according to the order of elution and outflow B-1 (20 g), B-2 (38 g) and several subsequent eluting aqueous ethanol fractions (total 230 g, water-ethanol 90:10→0:100 elution fraction).

[0044] Component B-1 (20g) was dissolved in an appropriate amount of water, directly applied...

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Abstract

The invention relates to a choerospondias extractive, a preparation method of phenol compounds contained in the choerospondias extractive, and the application of the extractive and phenol compounds in preparation of such functional foods as antihypoxia medicaments, antihypoxia health-care products, and the like.

Description

Technical field: [0001] The invention relates to the preparation method of the plant extract of the medicinal plant Jujube jujube and the phenolic acid compounds contained therein, and the use of these extracts and phenolic acid compounds to prepare functional foods such as anti-hypoxic drugs and anti-hypoxic health products the use of. Background technique: [0002] Oxygen is indispensable in human life activities, and oxygen molecules play an important role in the energy metabolism of tissues and cells in the body. A variety of factors can lead to insufficient oxygen supply to human tissues and cells, such as certain diseases or being in a special hypoxic environment (such as plateau), etc., and hypoxia can in turn cause cell and tissue damage and even various serious diseases, such as Severe respiratory diseases, concurrent cardiovascular and cerebrovascular diseases, and low immune function. Severe hypoxia is often the direct cause of death in many related critically i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K31/192A61K31/7024A61K36/22A61P43/00A61P9/00
Inventor 崔承彬李长伟蔡兵韩冰李明明范明
Owner INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A
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