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Collection and culture method of enteromorpha linza spore

A culturing method and a technology for spores of E. prolifera are applied in the field of collecting and cultivating spores of E. prolifera, which can solve the problems of not discussing a large number of methods for collecting zoospores, not proposing zoospores, etc. Strong, high-yield effects

Inactive Publication Date: 2009-12-30
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although when studying the life history of another common species of this genus, Enteromorpha prolifera, the researchers found that under the stimulation of light, a large number of zoospores were continuously released from its sporangia, but the existing The technology does not discuss the method of collecting a large number of zoospores, and there is no method and equipment to obtain a large number of zoospores continuously and stably

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0023] Sample collection: Mature Enteromorpha marginatus was taken and placed in sterile seawater for temporary cultivation.

[0024] Take about 2 cm of yellow-brown algae on a glass slide, add a small amount of sterilized seawater dropwise, and place it on a Nikon MD-TS100 microscope stage.

[0025] The spore collection test was divided into three groups: group A, light intensity 3500Lx; group B, light intensity 500-3000Lx; group C, control group, indoor natural light irradiation. The light time is 10 minutes, and seawater is added dropwise in time to prevent evaporation from drying up the algae. Microscopic examination, use a pipette to absorb the released spores, that is, to absorb them with a pipette, and place them in a 50ml small beaker for later use. Repeat the above st...

Embodiment 2

[0028] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0029] Sample collection: Mature Enteromorpha marginatus was taken and placed in sterile seawater for temporary cultivation.

[0030] Take about 2 cm of yellow-brown algae on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 5 minutes with a light intensity of 4000 Lx. Microscopic examination found that the zoospores were sucked up with a straw and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0031] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water, and dilute ...

Embodiment 3

[0036] The culture medium is natural seawater, and after the seawater is filtered, boiled and sterilized, and cooled to room temperature, it is placed in a high-temperature sterilized vessel for use.

[0037] Sample collection: Mature Enteromorpha marginatus was taken and placed in sterile seawater for temporary cultivation.

[0038] Take about 2 cm of yellowish-brown algae on a glass slide, add a small amount of sterilized seawater dropwise, place it on a Nikon MD-TS100 microscope stage, and stimulate it for 3 minutes with a light intensity of 4200 Lx. Microscopic examination found that the release of zoospores was sucked up with a straw, and placed in a 50ml small beaker for later use. Repeat the above steps to continue collecting spores 2 times. Replace with fresh algae fragments, and repeat the above steps to collect spores.

[0039] Take a small amount of spore liquid in a beaker and count it on a hemocytometer to determine the number of zoospores per milliliter of water,...

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Abstract

The invention discloses a collection and culture method of enteromorpha linza spore, including that mature enteromorpha linza spore fragment is placed on a glass slide and dropwise added with sea water, light with illumination intensity of 3500-4200Lx is used for irradiation and simulation, zoospores are collected when finding the zoospores are released, and then the zoospores are cultured. The invention can promote release of zoospores by high light irradiation of mature enteromorpha linza and can meet requirement of mass collection; the collection and culture method of the invention is simple and practical, has low requirement on frond maturity, not only steadily and continuously provides mass spores with normal growth and development capacity for experimental work, but also is convenient for observing appressorium and germination condition of spores on a large scale. The method provided by the invention fills the gap in enteromorpha linza spore collection and culture field, lays the foundation for artificial mass culture and has a wide application prospect in alga culture and prevention and removal test and research and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for collecting and cultivating Enteromorpha spores. Background technique [0002] According to the survey results of fouling organisms in various coastal sea areas of my country, it can be seen that the large algae such as Enteromorpha spp., Ulva, and Shuiyun are the dominant species of algae fouling organisms that are common in autumn, winter, and spring. The attachment of algae will significantly increase the dynamic load effect of waves and currents on marine facilities, causing drift or even overturning; blocking the mesh holes of aquaculture cages, cages, seines, etc., affecting the exchange of water in the internal and external environment, reducing the internal environment. Dissolved oxygen hinders the normal growth and development of cultured objects; chemical substances secreted by some algae may also have adverse effects on other marine organisms. [0003] Enteromor...

Claims

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Application Information

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IPC IPC(8): C12N5/04A01G33/00
CPCY02A40/80
Inventor 严涛谢恩义刘姗姗
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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