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Constructing method of ovary insulin resistant model

A technology of insulin resistance and construction method, applied in the field of construction of animal model and construction of ovarian insulin resistance model

Inactive Publication Date: 2011-05-18
吴效科
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Problems solved by technology

Obviously, it is inaccurate to explain the abnormal function of the ovary itself by the insulin resistance outside the ovarian tissue and the peripheral hyperinsulinemia.

Method used

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  • Constructing method of ovary insulin resistant model
  • Constructing method of ovary insulin resistant model

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Embodiment

[0019] The experimental animals were provided by the Experimental Animal Center of Heilongjiang University of Traditional Chinese Medicine. Female Kunming white mice (body weight (18±1) g / only) were selected as the experimental subjects. After the mice were sacrificed, the ovaries were removed for in vitro culture. After the mouse ovary was taken out, the surrounding tissue was peeled off under a microscope, placed in a 50mm petri dish containing physiological saline, and the ovary was washed with the prepared mixed solution of DMEM and 5% BSA for more than three times. Put four mouse ovaries into each group at random and carry out in vitro culture with F-12 medium, the composition of culture solution: the insulin of 5%BSA, 0.1%DMSO and 10nmol / l, wherein four groups add the dexamethasone of corresponding concentration, in Air humidity 95%, CO 2 cultured in a 5% incubator. Different concentrations (100nmol / L, 300nmol / L) of dexamethasone were used to induce mouse ovaries in vi...

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Abstract

The invention discloses a construction method of an ovary insulin resistant model, which belongs to the field of construction of an animal pathologic tissue model. The construction method comprises the following step: after a small mouse is killed, taking out the ovary and culturing the ovary by adopting an in vitro culture medium in a CO2 culture box for in vitro culture, wherein during the in vitro culture, hexadecadrol is added into the in vitro culture medium for induction. The ovary insulin resistant model is constructed by adopting the hexadecadrol to induce and culturing the ovary of the small mouse in vitro, and the change condition of the functions of the ovary in the insulin resistant model is evaluated by detecting the content of glucose in culture liquid. Shown by an experimental result, the effect of inducing ovary insulin to resist by the hexadecadrol has time dose dependency, and after the ovary is treated by the hexadecadrol, the intake of the ovary to the glucose is reduced. Under the stimulation of the insulin, the intake of the ovary treated by the hexadecadrol to the glucose is obviously reduced, thereby indicating that the ovary insulin resistant model is ideal.

Description

technical field [0001] The invention relates to a method for constructing an animal model, in particular to a method for constructing an ovarian insulin resistance model, and belongs to the field of constructing animal pathological models. Background technique [0002] Polycystic ovary syndrome (PCOS) is a common cause of infertility in women of reproductive age, characterized by abnormal ovarian function and associated with insulin resistance and compensatory hyperinsulinemia. But current research is limited to the classic target tissues of insulin action - skeletal muscle, fat and liver, etc. Insulin receptors are also present in ovarian tissue, suggesting that the ovary is another important target organ for insulin action. Obviously, it is inaccurate to explain the abnormal function of the ovary itself by the insulin resistance outside the ovarian tissue and the peripheral hyperinsulinemia. Therefore, it is necessary to construct a model of ovarian insulin resistance to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
Inventor 吴效科
Owner 吴效科