Hand-foot-and-mouth disease (HFMD) related virus parallel detection liquid-phase chip and preparation method and application thereof
A parallel detection and liquid phase chip technology, which is applied in the field of molecular biology technology and clinical detection, can solve the problems of inconvenience, tedious and time-consuming laboratory and clinical diagnosis, and achieve the effect of saving detection cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] Example 1: Coupling of probes to known numbered microspheres
[0027] 1. Select No. 26, No. 36 and No. 46 carboxyl microspheres (Luminex Company) respectively, and oscillate the microsphere suspension with a vortex shaker for 20 seconds to make the microspheres mix evenly.
[0028] 2. Take about 2×10 carboxyl microspheres of each number above. 3 Transfer each to a centrifuge tube and centrifuge at ≥8000×g for 2 min to precipitate carboxylated microspheres.
[0029] 3. Remove the supernatant and add 100μl dH 2 O, use a vortex shaker to resuspend the microspheres for 20s, centrifuge at ≥8000×g for 2min, and precipitate the carboxylated microspheres.
[0030] 4. Remove the supernatant, add 80 μl, 100 mM, pH = 6.2 sodium dihydrogen phosphate salt solution, and resuspend the washed carboxy microspheres with a vortex shaker for 20 seconds.
[0031] 5. Add 10μl, 50mg / ml Sulfo-NHS (with dH 2 O dilution), oscillate gently with a vortex shaker.
[0032] 6. Add 10μl, 50mg / ml ...
Embodiment 2
[0043] Example 2: Application of the liquid chip for parallel detection of hand, foot and mouth disease-associated virus according to the present invention in clinical detection
[0044] The technical process of detecting HFMD-related viruses by using the parallel detection liquid chip of HFMD-related viruses according to the present invention:
[0045] 1. Extraction of viral nucleic acid: RNA can be extracted using commercial kits, such as using QIGEN ViralRNA mini Extraction Kit (CAT: 52904) to extract viral RNA from clinical specimens or virus isolation cultures;
[0046] (1) Add 560 μl of AVL buffer to a 1.5ml centrifuge tube;
[0047] (2) Add 140 μl of clinical specimen or virus isolation culture to this centrifuge tube;
[0048] (3) Place at room temperature for 10 minutes, then centrifuge briefly;
[0049] (4) Add 560 μl of absolute ethanol, mix thoroughly for at least 15 seconds, and centrifuge briefly;
[0050] (5) Carefully add about 630 μl of the mixed solution t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com