Method for fast detection and identification of bacterial wilt caused by infection of ralstonia solanacearum

A technology for identification of solanaceae solanaceous wilt bacteria, which is applied in the field of rapid detection and identification of solanaceous wilt, can solve the problems of rare plant pathogenic microorganisms, no identification of solanaceae solanaceae, and achieves accurate, reliable and adaptable identification results. Strong, low-cost effects

Active Publication Date: 2010-03-17
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI +1
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Problems solved by technology

The identification process of the above two identification systems takes more than 18 hours, and it is necessary to purchase expensive imported special identification materials and equipment, and the system is mainly suitable for the identification of human, animal and environmental microorganisms, and is rarely used for the identification of plant pathogenic microorganisms , there is no report on the identification of Solanaceae bacterial wilt in the current literature

Method used

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  • Method for fast detection and identification of bacterial wilt caused by infection of ralstonia solanacearum
  • Method for fast detection and identification of bacterial wilt caused by infection of ralstonia solanacearum
  • Method for fast detection and identification of bacterial wilt caused by infection of ralstonia solanacearum

Examples

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Embodiment 1

[0032] Example 1: Detection and identification of known disease samples

[0033] 1. Experimental materials: Eggplant and tomato disease-like tissues caused by the infection of R. solanacearum and known healthy eggplant tissues were used as materials.

[0034] 2. Experimental method:

[0035] (1) Rinse the stem base and root of the diseased sample to be tested with water, and take a vascular bundle tissue with a size of about 1.5-2 mm × 2.5-3 mm from the stem base of the diseased sample to be tested, and 8 samples to be tested include 4 eggplants diseased sample and 4 tomato diseased samples, and another healthy eggplant sample was taken as a control;

[0036] (2) Use a sterile pipette to draw 100 μL of sterilized water, drop it on a clean glass slide, and use sterilized tweezers to put the diseased tissue into the 100 μL sterilized water drop on the clean glass slide. Forcefully press the diseased tissue into the water droplets, and let it stand for about 5 minutes; observe ...

Embodiment 2

[0042] Embodiment 2: detection and identification test to unknown disease sample

[0043] 1. Experimental materials: 10 different plant bacterial wilt samples or asymptomatic healthy samples collected from fields in multiple regions were tested.

[0044] 2. Experimental method:

[0045] 10 different plant samples collected from multiple regions in Guangdong were sampled, and a total of 17 samples were obtained and numbered. The plant species, collection locations, and symptoms corresponding to each number of samples are shown in Table 1. The method used for detection and identification is the same as that used in Example 1, and is compared and verified with the traditional method for isolation and identification of plant pathogenic bacteria.

[0046] The specific steps of the traditional method for isolation and identification of plant pathogenic bacteria include: 1. Disinfect the surface of the diseased tissue to be examined with 75% alcohol; Streak and culture at 28-30°C f...

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Abstract

The invention discloses a method for the fast detection and identification of bacterial wilt caused by the infection of ralstonia solanacearum. The method comprises three stages of preparing plant disease samples, observation with naked eyes and PCR verification, wherein the detection and the identification can be completed within four hours with low cost. The method makes a breakthrough on characteristics of traditional bacteriosis identification such as long period, high professional demand and higher cost and also overcomes the deficiencies of microbial identification system imported overseas, such as specific instruments and materials, high cost and longer identification period, and by means of the organic combination of special superficial symptoms of the disease sample and molecularbiotechnology, accurate and reliable results can be obtained in order to meet the demand of fast, accurate identification for plant diseases in actual production.

Description

technical field [0001] The invention belongs to the technical field of plant disease identification, in particular to a rapid detection and identification method for bacterial wilt caused by Ralstonia solanacearum infection. Background technique [0002] For the detection and identification of bacterial wilt caused by R. solanacearum infection of Solanaceae, there are three existing technologies: the traditional method for isolation and identification of plant pathogenic bacteria, the BIOLOG microbial identification system and the Mérieux microbial identification method. [0003] The traditional identification method of plant bacterial wilt mainly includes the steps of isolating pathogenic bacteria from disease samples, obtaining pure culture of isolated strains, observing the morphological characteristics of colonies and bacteria, measuring physiological and biochemical characteristics, and determining pathogenicity. It takes at least one month or more, the workload is heav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04G01N27/447C12R1/01
Inventor 何自福佘小漫虞皓罗方芳
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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