Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim

A technology of trichomoniasis group and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as slow reproduction speed, large blindness, and imbalance of male and female ratios, so as to reduce pests and diseases of Trichosanthes chinensis and save costs The effect of consistent investment and harvest period

Inactive Publication Date: 2010-05-05
杨保成
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seed planting, blindness is large, it is difficult to obtain the desired product, and it is easy to cause an imbalance of male and female ratios (Tachia chinensis is dioecious, female plants mainly bear fruits, male plants mainly grow trichosanthes, and in the flowering stage The external forms of the former t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] A method for rapid propagation of Trichosanthes chinensis tissue culture, which is through the configuration of medium, the picking of explants and the establishment of primary culture, subculture multiplication culture, rooting culture of strong seedlings, transplanting of test-tube seedlings, field transplanting six carried out in steps, characterized in that:

[0029] 1. Configuration of culture medium

[0030] ① Prepare the mother solution required for the MS basic medium, and take the specified amount according to the different times of the mother solution. After adding the mother liquor, add the required plant growth regulating substances through calculation. After adding, pour into the melted agar, then add sucrose, wait for the sucrose to dissolve, adjust the volume to the required volume, continue to heat and stir until the agar is completely dissolved. Adjust the pH value of the solution to 5.8-6.2 with a pH meter or pH test paper.

[0031] ② Dispense the p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the traditional Chinese medicine biotechnological field, in particular to a method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim, including six steps of configuration of culture medium, picking of explant and establishing of initial generation culture, successive generation propagation culture, strong stock rooting culture, transplantation of seedling in test tube and transplantation to land for growing field crops. By adopting the technical scheme of the invention, (1) as the seedling is completely deviated from the same plant, breed and hereditary characteristics are all the same, thus being convenient for rapid implantation propagation of improved breed; (2) growth of seedling is rapid and tidy and harvest period is almost the same, thus being beneficial to intensive management and commercialization production; (3) plant diseases and insect pests of Trichosanthes kirilowii Maxim are greatly reduced, and cost investment is reduced; (4) by tissue culture and optimization, yield per mu of fresh Trichosanthes kirilowii Maxim fruit can reach more than 5000 kilo grams (increased by 2500-3500 kilo grams), yield of dry Trichosanthes kirilowii Maxim fruits can reach more than 1000 kilo grams (increased by 500-600 kilo grams), and yield of radices trichosanthis can reach more than 1000 kilo grams (increased by 500-600 kilo grams).

Description

technical field [0001] The invention relates to the field of biotechnology of traditional Chinese medicinal materials, in particular to a method for rapid propagation of Trichosanthes chinensis tissue culture. Background technique [0002] At present, the field propagation of Trichosanthes is mostly planted by seeds or tubers. Seed planting, blindness is large, it is difficult to obtain the desired product, and it is easy to cause an imbalance of male and female ratios (Tachia chinensis is dioecious, female plants mainly bear fruits, male plants mainly grow trichosanthes, and in the flowering stage The external forms of the former two are very similar and indistinguishable); root tuber planting is easy to control male and female, but it wastes a lot of trichosanthes medicinal materials that could be used for sale, resulting in a great waste of resources and a sharp decrease in the income of drug farmers, and the speed of reproduction slow. Therefore, we urgently need a new...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 杨保成
Owner 杨保成
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap