Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for calibrating and measuring activity of 5'-nucleotidase, matching kit and application thereof

A nucleotidase and kit technology, applied in the field of medical testing and determination, can solve problems such as inappropriate clinical biochemical testing, and achieve the effect of improving testing accuracy

Active Publication Date: 2010-05-19
NINGBO RUI BIO TECH
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the 5'-nucleotidase dehydrogenase method can quantitatively measure 5'-nucleotidase activity, but this method is easily interfered by exogenous ammonia and is not suitable for clinical biochemical tests

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for calibrating and measuring activity of 5'-nucleotidase, matching kit and application thereof
  • Method for calibrating and measuring activity of 5'-nucleotidase, matching kit and application thereof
  • Method for calibrating and measuring activity of 5'-nucleotidase, matching kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] The following are specific examples of the present invention to further describe the technical solutions of the present invention, but the present invention is not limited to these examples.

[0042] 1. Determination of 5'-nucleotidase standard and its activity

[0043] (1) 5'-nucleotidase standard product activity 0-100U / L human gene recombinant 5'-nucleotidase

[0044] (2) Determination of 5'-nucleotidase standard activity

[0045] Reagent composition:

[0046] Reagent RI: including 10mol / L 5'-inosine nucleotide, 0.015mol / L a-ketoglutarate, 0.30×10 -3 mol / L reduced coenzyme I disodium salt, and 0.10mol / L phosphate buffer solution with a pH value of 7.20;

[0047] Reagent RII: glutamate dehydrogenase at 200 U / ml.

[0048] Biological samples: 0-100U / L human gene recombinant 5'-nucleotidase

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for calibrating and measuring the activity of 5'-nucleotidase and a matching kit. In the invention a 5'-nucleotidase dehydrogenase removal method is used to detect the kit for determining the activity of a standard product of the 5'-nucleotidase and then a Trinder' method is applied to quantitatively measure the activity of the 5'-nucleotidase in a biologic sample by taking the activity of the standard product as a reference. The invention has the advantages of solving the defect of the Trinder' method, enhancing the accuracy, reducing the instrument error and reinforcing the data comparability of the normal reference range of the 5'-nucleotidase, is used for the diagnosis and the like of the diseases of primary and transferable liver cancers, biliary tract cancers, pancreatic cancers, biliary tract obstruction, cholangitis, acute and chronic hepatitis, hepatocirrhosis, medicinal hepatic injury and the like by measuring the activity of the 5'-nucleotidase and is suitable for popularization and use in hospitals.

Description

technical field [0001] The invention belongs to the technical field of medical examination and measurement, and in particular relates to a method for calibrating and measuring 5'-nucleotidase, a matched kit and its application. Background technique [0002] In the prior art, it is more common to use Trinder's method to quantitatively measure 5'-nucleotidase activity in clinical chemistry tests. However, the disadvantage of Trinder's method is that it cannot use a recognized standard method to determine the 5'-nucleotidase activity. Enzyme standard products, resulting in a decrease in test accuracy, instrument errors caused by different biochemical analyzers used in different laboratories, and also the main reason for the confusion and incomparability of the normal reference range data for 5'-nucleotidase. [0003] 5'-nucleotidase dehydrogenase method uses 5'-nucleotides to enzymatically hydrolyze 5'-inosine nucleotides to generate inosine and ammonia, ammonia and α-ketogluta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/42G01N21/31
Inventor 张闻
Owner NINGBO RUI BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com