Molecular specificity mark primer for authenticating mushroom strains 241 and 241-4 and detecting method

A technology of labeling primers and specificity, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc. Difficult to achieve fast and accurate identification and other problems, to achieve the effect of short detection time, high accuracy and simple method
CN101712984AInactive Publication Date: 2010-05-26ZHEJIANG FORESTRY ACAD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG FORESTRY ACAD
Publication Date
2010-05-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a molecular specificity mark primer for authenticating mushroom strains 241 and 241-4 and a detecting method for differentiating and authenticating the mushroom strains 241 and 241-4 by using same. The sequence of an upstream primer is 5'-GGTTCTGCTCCTTGTGACTG-3', and the sequence of a downstream primer is 5'-TCTGCTCTTCAGATGCAAGCT-3'. Compared with convectional morphology detection, antagonism tests and fruiting tests, the invention has the advantages of short detection time and high accuracy. The detection time in the method is only 1 to 2 days, but the time of the convectional antagonism tests is at least two weeks, and the time of the fruiting tests is at least three months.
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Description

(1) Technical field

[0001] The invention relates to a molecular-specific marker primer capable of distinguishing mushroom-producing close relatives 241 and 241-4, and a method for distinguishing and identifying mushroom-producing strains 241 and 241-4 using the primer. (2) Background technology

[0002] Lentinula edodes (Berk.) Pegler is loved by domestic and foreign consumers because of its delicious taste and important food and medicinal value. It is an edible fungus with the largest commercial production scale in my country and second only to Agaricus bisporus in the world. . China is the first country to cultivate shiitake mushrooms, and the current output of shiitake mushrooms has reached 70% of the world.

[0003] Lentinus edodes strains are the most important means of production in the production of shiitake mushrooms. At present, there are more than 100 kinds of shiitake mushrooms used in commercial cultivation in China. Different shiitake mushroom strains have diff...

Claims

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