Cultivation method of black-peel chewing cane healthy tissue-cultured cup seedlings planted in early spring for coming into market in winter
A cultivation method and tissue culture technology, applied in the field of high-yield cultivation of black-skinned fruit cane healthy tissue culture cup seedlings planted in early spring and listed in winter, can solve the problem of complex production conditions in sugarcane areas, poor isolation of virus-free healthy seedlings, Problems such as high probability of re-susceptibility, to achieve the effect of shortening the planting time
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[0014] The present invention will be further described in detail through the following specific examples, but the variety and planting time of the fruit cane of the present invention are not limited thereto.
[0015] 1. Donor material selection
[0016] From June to July every year, select the tip part of the black fruit cane that grows healthy, free from diseases and insect pests, and has the characteristics of this variety. After removing the excess leaves, disinfect it with 75% alcohol; or take its side buds and treat them with hot water for detoxification. Finally, carry out thermal cultivation, and when the false height of stem seedlings is 15 cm, get strong seedlings, and after removing redundant leaves, disinfect with 75% alcohol.
[0017] 2. Production of black-skinned fruit cane tissue culture seedlings
[0018] Under sterile conditions, based on MS medium (MS is the most commonly used medium name for plant tissue culture, the main components are various chemical sub...
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