Novel method for lowering breath resistance of bronchial asthma

A bronchial asthma and respiratory resistance technology, applied in the field of pathology, can solve the problems of unable to simulate the real situation, unable to provide MLCK function, unable to draw clear conclusions, etc., to improve the symptoms and course of asthma, reduce respiratory resistance, bronchial respiratory resistance reduced effect

Inactive Publication Date: 2010-06-16
NANJING UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

As we all know, this correlation study cannot provide direct evidence on the function of MLCK, and in

Method used

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  • Novel method for lowering breath resistance of bronchial asthma
  • Novel method for lowering breath resistance of bronchial asthma
  • Novel method for lowering breath resistance of bronchial asthma

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0014] The present invention is described further below by embodiment.

[0015] 1. Construction of airway smooth muscle-specific knockout MLCK mice

[0016] First, the kinase domain of MLCK was removed from the BAC clone bMQ 366n04 by vector transformation technology, and then a Lox P sequence and a positive selection marker NeoFRT sequence were inserted on both sides of the 23-25 ​​exons encoding the ATP binding site. Then the modified vector was linearized, electroporated into mouse embryonic stem cells to recombine with the alleles of the genome, positive clones were identified by PCR and Southern, and then the positive clones were injected into the blastocysts of pseudopregnant mother mice , so that they give birth to chimeric mice. The mouse was compared with the mouse SM-CreER that specifically expresses Cre recombinase in smooth muscle T2 (ki) mating, the positive mice born grow to 2-3 months, and 1 mg of tamoxifen drug (Sigma company) is injected intraperitoneally fo...

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Abstract

The invention discloses a novel method for lowering the breath resistance of the bronchus, which achieves the aim of treating asthma by the function of suppressing MLCK and still belongs to the first time at home and even abroad. The preparation of an MLCK gene knockout mouse and the research of the contraction function of the MLCK gene knockout mouse confirm the key function of the MLCK in the contraction of the smooth muscle of the air duct so as to confirm that the breath resistance of the bronchus can be obviously lowered under the conditions of physiology and pathology by suppressing the MLCK, thereby making a sufficient preparation for the next artificial synthesis of an MLCK suppressor used for treating the asthma. The invention not only finds the novel method for lowering the breath resistance of the bronchus but also provides a concept for developing other treatment schemes afterwards.

Description

technical field [0001] The present invention relates to the field of pathology. Specifically, the present invention achieves the purpose of reducing the respiratory resistance of bronchial asthma by inhibiting the activity of MLCK molecules. Background technique [0002] Bronchial asthma is a disease that seriously endangers the health of the population. About 200 million people in the world suffer from asthma. The statistical incidence rate varies from country to country, about 1%-5%, of which children and women account for an important proportion. With the process of industrialization and the intensification of environmental pollution, the incidence of asthma and the probability of complications are on the rise. Asthma has become a serious social problem and has attracted the attention of countries all over the world. [0003] Asthma is not a single disease but a syndrome of multiple pathogenesis producing the same symptoms. Asthma is an ongoing occurrence in atopy, and...

Claims

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Application Information

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IPC IPC(8): A61M5/178A61K31/138A61P11/06
Inventor 张文程何伟奇朱敏生
Owner NANJING UNIV
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