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Method for manufacturing tissue array paraffin block

A technology of tissue chip and production method, which is applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of low slice rate, fragmentation of acceptor wax block, complex structure, etc., and achieve simplified operation steps, reduce production cost, and improve the effect of film yield

Active Publication Date: 2012-07-04
苏州新芯生物技术有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tissue chip production process is prone to problems such as fragmentation of the receptor wax block and tissue sample drift, lodging, and displacement. The instrument is not only complex in structure but also expensive, which to a large extent restricts the development, promotion and popularization of tissue chip technology

Method used

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Embodiment Construction

[0019] The present invention will be further described below in combination with specific embodiments.

[0020] According to the technical scheme of the present invention, a method for making a paraffin tissue chip wax block uses tissue chip glue to make the tissue core adhere to the same plane of the embedding bottom mold, after paraffin embedding and cooling, the tissue chip is made with a release agent. The glue loses its viscosity, thereby obtaining the tissue microarray block. Including the following steps:

[0021] 1) Sampling: Warm the donor wax block to 35°C-45°C for more than 15 minutes, use the sampling end of the disposable sampling needle to sample, and put the needle cap on the sampling end of the disposable sampling needle.

[0022] 2) Arrangement: Arrange the disposable sampling needles for taking organized cores one by one on the nipple array of the positioning cooler (the tissue sequence of the finally made wax block is consistent with it), and save all the s...

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Abstract

The invention discloses a method for manufacturing a tissue array paraffin block, which comprises the following steps: attaching the tissue array to the same plane of an embedding bottom mold by using tissue array adhesive, embedding paraffin, cooling, and reducing the adhesion strength of the tissue array by using a releasing agent, thereby obtaining the tissue array paraffin block. The method comprises the steps of sampling, arranging, array planting, paraffin filling and demolding. The invention is applied to the techniques for manufacturing paraffin tissue arrays in the field of biochips.The method can be used for manufacturing the tissue array paraffin blocks with ease and high efficiency.

Description

technical field [0001] The invention relates to a method for making a biological chip, in particular to a method for making a paraffin tissue chip wax block. Background technique [0002] Tissue chips (tissue chips, TCs), also known as tissue microarrays (tissue microarrays, TMAs), like gene chips, protein chips, and cell chips, are an integral part of biochip technology and belong to high-tech biotechnology. Tissue chip is a new type of high-throughput, multi-sample research tool that arranges dozens or hundreds of tissue samples of different individuals on a carrier according to a predetermined sequence for morphological or molecular biology experiments. [0003] Because tissue microarray technology has the advantages of large amount of information, small experimental error, strong comparability, time saving, labor saving, and a lot of money saving, it is compatible with traditional pathological technology, histochemical technology, immunohistochemical technology (IHC), an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/48
Inventor 李从善
Owner 苏州新芯生物技术有限公司
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