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Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum

A fluorescence spectrum and flow cell technology, applied in the field of chemical testing and analysis equipment, can solve problems such as application difficulties, and achieve the effect of small size, light structure, and simultaneous detection and spectral observation.

Inactive Publication Date: 2010-07-14
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Restricted by the above factors, it is difficult to apply the common online analytical instruments or liquid chromatography UV-visible absorption detectors and fluorescence detectors in portable analytical instruments.

Method used

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  • Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum
  • Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum
  • Flow cell device suitable for synchronous on-line detection of absorption spectrum and fluorescence spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The flow cell body device for synchronous online detection of absorption photometry and fluorescence photometry is composed of a light source spectrum fiber interface hole (2), an ultraviolet-visible light fiber interface hole (1), a fluorescence spectrum fiber interface hole (5), and a liquid inlet tube hole ( 3) A five-way connection formed by the liquid outlet pipe hole (4), wherein the diameter of the linear channel between the ultraviolet-visible optical fiber interface hole (1) and the light source spectrum optical fiber interface hole (2) is 0.6 mm; the liquid inlet The pipe hole (3) and the liquid outlet pipe hole (4) are arranged side by side, and the distance between the centers of the liquid inlet pipe hole (3) and the liquid outlet pipe hole (4) is 1 cm; the fluorescence spectrum optical fiber interface hole (5) and the light source Spectrum optical fiber interface holes (2) are vertically distributed at right angles; the inner diameter of the hole is thicken...

Embodiment 2

[0029] The flow cell body device The cell body is integrally processed from polyimide materials such as polytetrafluoroethylene, and the selected material has the characteristics of acid resistance, alkali resistance and high temperature resistance. The linear channel between the ultraviolet-visible optical fiber interface hole (1) and the light source spectrum optical fiber interface hole (2) is located along the central axis of the pool body, the channel diameter is 0.5mm, the inner diameter of both ends of the hole is thickened and processed with optical fiber connectors The matching internal thread is used to connect and fix the optical fiber on the cell body of the flow cell device with a joint and a ferrule.

[0030]Because the flow cell body device provided above has simple structure design, small volume, flexible configuration and easy movement, it is widely used in flow injection analysis or high-performance liquid chromatography dedicated absorption-emission (fluoresc...

Embodiment 3

[0032] The flow cell device cell body is integrally processed by polyimide materials such as polytetrafluoroethylene, and the linear channel between the ultraviolet-visible optical fiber interface hole (1) and the light source spectrum optical fiber interface hole (2) is located along the cell body The axis and channel diameter are 0.7mm. The inner diameters of both ends of the hole are thickened and processed with internal threads that match the optical fiber connectors. Connect and fix the optical fiber on the flow cell device cell body with connectors and ferrules.

[0033] Because the flow cell body device provided above has simple structure design, small volume, flexible configuration and easy movement, it is widely used in flow injection analysis or high-performance liquid chromatography dedicated absorption-emission (fluorescence) synchronous spectrum detection cell, especially the liquid inlet tube The center-to-center distance between the hole and the liquid outlet pip...

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PUM

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Abstract

The invention discloses a flow cell, which is a five-way body consisting of a light source spectrum fiber interface hole, an ultraviolet-visible light fiber interface hole, a fluorescence spectrum fiber interface hole, a liquid inlet pipe hole and a liquid outlet pipe hole, wherein the diameter of a linear passage pipe between the ultraviolet-visible light fiber interface hole and the light source spectrum fiber interface hole is 0.5 to 0.7 millimeter; the liquid inlet pipe hole and the liquid outlet pipe hole are distributed in parallel, and the central distance of the two holes is 1 centimeter; the fluorescence spectrum fiber interface hole and the light source spectrum fiber interface hole are distributed in a vertical right angle; the inside diameters of the holes are thickened, and matched inner threads are machined in the holes; and a fiber or a pipeline is connected and fixed to the cell via a joint and a buckling sleeve. The flow cell can be widely applied to the field of a flow cell device applicable in the ultraviolet-visible light absorption and fluorescence detection on a flow injection analysis instrument.

Description

technical field [0001] The invention belongs to the field of equipment for chemical testing and analysis. Specifically, the present invention relates to a component for an on-line analysis instrument, in particular to a flow cell body device for synchronous on-line detection of absorption spectrum and fluorescence spectrum in a portable on-line analysis instrument or a high-performance liquid chromatography detection instrument. Background technique [0002] In scientific research and production work, it is often necessary to detect absorption and emission spectra simultaneously. UV-Vis absorption detectors or fluorescence detectors are the two most common detectors found on on-line analytical instruments or HPLC instruments. The flow cell assembly is an important component in both UV-Vis absorption detectors and fluorescence detectors. After the light from the light source in the on-line analytical instrument or high-performance liquid chromatography UV-visible absorption...

Claims

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Application Information

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IPC IPC(8): G01N21/05G01N21/33G01N21/64G01N30/62
Inventor 艾尔肯·依布拉音李明陈坚
Owner XINJIANG MEDICAL UNIV
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