Kit for detecting beta2-microglobulin by nanometer microsphere immunoturbidimetry

Abstract

The invention relates to a kit for measuring beta2-microglobulin in serum. The technical problem to be solved is to overcome the disadvantages of background technology and provide a kit for detecting the beta2-microglobulin by nanometer microsphere immunoturbidimetry, which has no need of dilution of a sample, simple operation, high accuracy and good repeatability, and is applicable to full automatic biochemical analyzers with diverse types. The technical scheme comprises that: the kit for detecting the beta2-microglobulin by the nanometer microsphere immunoturbidimetry comprises a reagent R1, a reagent R2 and a reference calibration material, wherein the reagent R1 comprises buffer solution, a preservative, a stabilizing agent, an electrolyte, a surfactant and the balance of purified water; the reagent R2 comprises the buffer solution, nanometer microspheres which is combined with an anti-human beta2-microglobulin antibody and has a diameter of between 50 and 150 nm and the preservative; and the reference calibration material comprises the buffer solution, the stabilizing agent, the preservative, a recombinant human beta2-microglobulin pure product in a proper amount determined by concentration requirement and the balance of the purified water.

Description

technical field The invention relates to a test kit for measuring serum components, in particular to a test kit for measuring β2-microglobulin (β2-MG) in serum, which can be widely used in the technical fields of medicine and biochemistry. Background technique beta 2 - Microglobulin (β 2 -MG) is a low molecular weight (11800) protein that was first isolated in the urine of patients with renal tubular nephritis; β 2 -MG exists on the surface of almost all nucleated cells and is a component of the light chain of human leukocyte antigen (HLA) molecules. As a product of HLA metabolism and breakdown, β 2 -MG occurs in low concentrations in free form in serum, urine, and other body fluids. free beta 2 -MG is excreted and degraded through glomerular filtration tubular reabsorption. because beta 2 -MG has a relatively constant production rate in the body every day and is only excreted by the kidneys. Determination of blood β2-MG can be used as an indicator of glomerular filtr...

AI Technical Summary

Problems solved by technology

These methods have the disadvantages of cumbersome operation, special equipment, sample pretreatment, batch sample analysis and automatic biochemical analyzer detection.

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