Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel use of lignan-type compounds or extract of nutmeg or aril of nutmeg comprising the same

An extract, the technology of nutmeg, which is applied to the field of new application of lignan-based compounds or nutmeg extract or nutmeg aril extract containing the compound, can solve the problem of ignorance of whitening activity, etc.

Active Publication Date: 2010-09-08
AAT COSTECH CO LTD +1
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not known whether or not osbilignan 7 or ricalin E extracted from the aforementioned nutmeg or macee aril including macelignan has whitening activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel use of lignan-type compounds or extract of nutmeg or aril of nutmeg comprising the same
  • Novel use of lignan-type compounds or extract of nutmeg or aril of nutmeg comprising the same
  • Novel use of lignan-type compounds or extract of nutmeg or aril of nutmeg comprising the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Preparation of Nutmeg or Nutmeg Aril Extract with Whitening Activity

[0072] Preparation of Nutmeg Extract

[0073] First, in order to prepare ethanol extract of nutmeg, dry nutmeg was pulverized with a mixer, then 100 g of pulverized nutmeg sample was added to 1 L of ethanol, and cold soaked at room temperature for 48 hours for extraction. The extracted sample was filtered with Whatman No. 2 filter paper, and then the filtered extract was concentrated by a vacuum rotary concentrator to remove solvent components, thereby obtaining an ethanol extract of nutmeg.

[0074] Using methanol, hexane or chloroform instead of ethanol as the extraction solvent and using the same method as above, the methanol, hexane or chloroform extract of nutmeg was obtained.

[0075] A supercritical extract of nutmeg was also prepared using a supercritical fluid extraction machine. Specifically, it is produced by using carbon dioxide as a supercritical fluid and removing the solvent from th...

Embodiment 2

[0079] Isolation of compounds with whitening activity from extracts of nutmeg or macee aril

[0080] Isolation of macelignan

[0081] The ethanol extract of nutmeg ethanol-concentrated in the aforementioned was mixed with ethyl acetate to extract ethyl acetate-soluble components, and ethyl acetate was removed to concentrate ethyl acetate-soluble components. In a 6x15cm column filled with silica gel, the solvent system obtained by mixing hexane and ethyl acetate in a ratio of 10:1 (v / v) was used to remove inactive substances and the like, followed by hexane and ethyl acetate in a ratio of 20: 1 (v / v) mixed solvents to obtain each fraction, use Rp-18 column chromatography to elute with 80% methanol, then dry the concentrated solution, and finally isolate the pure single whitening active substance that is the following chemical formula 1 Macelignan (4-((2S,3R)-4-benzo[1,3]dioxol-5-yl-2,3-dimethyl-butyl)-2-methanol oxyphenol).

[0082]

[0083]

[0084] Isolation of Osc...

experiment example 1

[0093] Melanogenesis inhibitory activity

[0094] Melanin Production Inhibition of Nutmeg or Mace Aril Extract

[0095] First, it was confirmed whether or not the ethanol extract of nutmeg prepared in the aforementioned had melanin production inhibitory activity.

[0096] Specifically, with 2×10 5 Cells / ml Melan-a cells were added to a 6-well plate (melanin quantification) (final volume: 3ml) with 10% FBS and 2 μM TPA (12-myristate-13-acetate-phorbol ester (phorbol 12- myristate 13-acetate)) of RPMI1640 in 10% CO 2They were cultured at 37° C. for 24 hours in an incubator (MCO-20AIC, Sanyo). The culture medium after the aforementioned cultivation was removed, and the ethanol extract of nutmeg prepared in the aforementioned was added to the RPMI 1640 medium, and treated every day for a period of three days. After 5 days, the medium cultured in the 6-well plate was removed, and treated with 0.25% trypsin-EDTA (SigmaChemical Co.) solution to recover the cell pellet (pellet)...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a new use of lignan-type compounds or an extract of nutmeg or aril of nutmeg comprising the same. More particularly, the present invention relates to a composition for skin whitening comprising a lignan-type compound or an extract of nutmeg or aril of nutmeg comprising the same. Because the lignan compound represented by Chemical Formulas 1 to 3 or the extract of nutmeg or aril of nutmeg has superior skin whitening activity by inhibiting melanin production and tyrosinase activity, it can be used for the preparation of a cosmetic composition, food composition or pharmaceutical composition for skin whitening.

Description

technical field [0001] This application claims Korean Patent Application No. 2007-0104783 filed on October 17, 2007 as the basis of priority, and the entirety of the foregoing specification is a reference document of the present invention. [0002] The present invention relates to a new application of a lignan compound or a nutmeg extract or nutmeg aril extract containing the compound. Specifically, the present invention relates to a whitening composition characterized by containing a lignan compound or a nutmeg extract or aril extract of nutmeg containing the compound. Background technique [0003] Although human skin color is determined by various factors such as the activity of melanocytes that produce melanin, the distribution of blood vessels, the thickness of the skin, and whether it contains carotenoids, bilirubin, etc. The black pigment called melanin produced by the action of various enzymes such as acidase is the most important. [0004] The formation of the afor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/49
CPCA61K8/4973A61K8/97A61Q19/02A61K31/357A61K36/185A61K8/9789A61P17/16A61P43/00A61K8/49
Inventor J-K·黄Y-M·曹G·H·金J·H·金H·J·李H·I·吴H·C·郑J·Y·郑D·U·金
Owner AAT COSTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com