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Method for preparing sweet potato solid culture medium

A technology of solid culture medium and culture medium, which is applied in the field of plant tissue culture and sweet potato tissue culture, can solve problems such as carrageenan national invention patents that have not yet been seen, and achieve the effects of reducing costs, reducing energy consumption, and strong coagulation

Inactive Publication Date: 2010-09-15
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a national invention patent for "the application of carrageenan as a coagulant for artificial feed for insects (Patent No. CN 02115104.0)" in my country, and there is no national invention patent for carrageenan as a coagulant for sweet potato tissue culture medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation process of the present embodiment sweet potato solid medium comprises the steps:

[0026] (1) Preparation of basal medium: according to the formula of MS medium, measure the mother liquor and mix it in proportion to prepare basal medium;

[0027] (2) Preparation of complete medium; add 3% sucrose to the basal medium of step (1) to prepare MS complete medium;

[0028] (3) Addition of coagulant: Add 4 g / L carrageenan to the MS complete medium prepared in step (2), and after the medium is fixed to 1 L with deionized water, the culture medium is cultured with 0.1M NaOH and 0.1M HCl The base pH value was adjusted to pH 5.8;

[0029] (4) Stir and mix the culture medium at room temperature, directly divide and seal, and sterilize by high-pressure steam at 103.4kPa and 121°C for 20 minutes to prepare the desired sweet potato solid culture medium.

[0030] The sweet potato solid medium prepared in this example has a low coagulation degree and is semi-solid and ...

Embodiment 2

[0032] The preparation process of the sweet potato solid medium in this embodiment is the same as in Example 1, but the concentration of carrageenan is 8g / L.

[0033] The obtained sweet potato solid medium prepared in this example has strong coagulation degree and high transparency, and is suitable as a solid medium for sweet potato tissue culture.

[0034] After the sweet potato seedlings grew on this medium for 30 days, the number of new leaves was 9.50±0.56, the plant height was 8.43±0.38cm, and the number of roots was 11.50±1.15; After 30 days of sweet potato seedlings, the number of new leaves was 8.83±0.48, the plant height was 7.93±0.51cm, and the number of roots was 4.17±0.65.

Embodiment 3

[0036] The preparation process of the sweet potato solid medium in this embodiment is the same as in Example 1, but the concentration of carrageenan is 10g / L.

[0037] The obtained sweet potato solid medium prepared in this example has strong coagulation degree and high transparency, and is suitable as a solid medium for sweet potato tissue culture.

[0038] After the sweet potato seedlings grew on this medium for 30 days, the number of new leaves was 8.83±0.54, the plant height was 8.07±0.95cm, and the number of roots was 8.83±0.76; After 30 days of sweet potato seedlings, the number of new leaves was 8.83±0.48, the plant height was 7.93±0.51cm, and the number of roots was 4.17±0.65.

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Abstract

The invention relates to a method for preparing a sweet potato solid culture medium. In the specific method, improvement is carried out on the basis of the preparation of the culture medium for the traditional sweet potato tissue culture, carrangeenan is used as a coagulant and replaces agar to be added into the culture medium of the sweet potato, and the used concentration of the carrangeenan is optimized and adjusted. In the preparation method, the coagulant is not needed to be heated and boiled for melting in the preparation process, the prepared culture medium has the advantages of high coagulability, high water holding property, stable pH value before and after sterilization, and the like. Compared with the agar which is used as the coagulant of the culture medium for the sweet potato tissue culture, the invention has no obvious influence on the growth and propagation of the sweet potato seedlings in test tubes, while the cost of the culture medium is decreased by 40%-50%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the technical field of sweet potato tissue culture, in particular to a method for preparing a sweet potato solid medium. Background technique [0002] Sweet potato tissue culture refers to the induction of callus and regeneration of complete plants from explants such as sweet potato leaves, petioles, stem segments, tubers, shoot tips, anthers, ovaries, and ovules. The main applications of sweet potato tissue culture are as follows: (1) Rapid propagation of fine varieties; (2) Virus-free cultivation of sweet potato shoot tips to cultivate disease-resistant varieties; (3) Preservation of original sweet potato variety resources; (4) Anther culture acquisition single Ploid plants are doubled into pure lines, sexual hybridization or grafting and cell hybridization are carried out, and high-yield and high-quality hybrids are purposefully cultivated. [0003] At present, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 高峰黄成涛周伟郭炜绵
Owner SOUTH CHINA NORMAL UNIVERSITY
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