Process for industrialized tissue culture and rapid propagation of high-bush blueberry

A rapid propagation and high irrigation technology, applied in the field of bioengineering, can solve the problems of long induction time, low survival rate, low induction rate of emergence, etc., and achieve the effect of convenient induction and cultivation and good effect

Inactive Publication Date: 2010-09-22
山东高端蓝莓生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art generally does not uniformly use a single medium for blueberry varieties, resulting in a low emergence induction rate; and the induction time is long, rooting is difficult, the survival rate is low, and it cannot adapt to faster market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.

[0028] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 10 minutes, and put it into a sterile bottle for later use after disinfection.

[0029] 2. Induce germination: Inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 0.8 mg / L + naphthalene acetic acid 0.2 mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 40 days, the buds grow into new sh...

Embodiment 2

[0032] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.

[0033] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 20 minutes, and put it into a sterile bottle for later use after disinfection is completed.

[0034] 2. Induce germination: inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 1.0mg / L + naphthaleneacetic acid 1.0mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 45 days, the buds grow i...

Embodiment 3

[0037] 1. Treatment of explant materials: including cleaning, disinfection and cutting of explant materials to prepare explants that meet the requirements.

[0038] The specific method is: use the tender stems of the current year with the leaves cut off, rinse them with tap water, rinse them with sterile water 3-5 times on a sterile workbench, rinse them with tap water for 20-30 minutes, and then soak them in water with detergent Wash for 20-30 minutes. After cleaning, carry out disinfection treatment on the ultra-clean workbench, put it into 0.1% mercuric chloride disinfectant solution with activated carbon for 30 minutes, and put it into a sterile bottle for later use after disinfection is completed.

[0039] 2. Induce germination: Inoculate the explants in a medium formulated as agar comprehensive medium + zeatin 1.3mg / L + naphthaleneacetic acid 1.6mg / L, inoculate the explants on the induction medium, and start after 1 week After germination, after 52 days, the buds grow i...

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PUM

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Abstract

The invention provides a process for the industrialized tissue culture and rapid propagation of high-bush blueberry. The process comprises the following steps: (1) treating explants materials, i.e., selecting and treating explants materials, and preparing explants satisfying the requirements at the balanced point of the minimal killing rate and contamination rate; (2) inducing, i.e., inoculating the explants in step (1) on a culture medium, and inducing the explants to bud under the condition of inducing, wherein the culture medium is particularly an improved agar synthetic medium; and (3) subculturing, i.e., carrying out the enrichment culture on the shoots of the cluster buds obtained in step (2) in a subculture medium.

Description

Technical field: [0001] The invention belongs to the technical field of bioengineering, and relates to plant tissue culture technology, in particular to a process flow for industrialized tissue culture and rapid propagation of high-shrub blueberries. Background technique: [0002] High bush blueberry (Blueberry), scientific name lingonberry, is a perennial shrub native to North America. Generally, seeds cannot be formed, and cuttings, layering and other propagation methods are mostly used. However, high-shrub blueberries have strict requirements on temperature, soil, and climate. , the quality of cutting propagation is not ideal. The technology of tissue culture propagating plants is a special vegetative propagation method, which is now generally called rapid propagation technology or micropropagation technology. It is a method of propagating plants under sterile conditions by using part of the plant body, including bacteria, tissues and organs, under artificially controll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 山东高端蓝莓生物技术股份有限公司
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