Kit for enzymatic determination of 1,5-anhydro-D-glucitol

Abstract

The invention discloses an reagent for enzymatic determination of 1,5-anhydro-D-glucitol. At present, determination operation has a lot of trouble and is difficult to be detected in bulk, and in the methods for the operation, the reagent only can be prepared when being used without long term preservation. The reagent of the invention comprises the following components with the contents in 1 liter of water: 60-140 mmol of 3-hydroxyl methyl amino methane, 2.26-2.38 mmol of oxidized coenzyme II (NADP+), 1-5 grams of trehalose, 0.1-0.6 activity unit of 2-methyl-4-isothiazole-3-ketone, 0.1-0.6 gram of Twain, 1000-3000U of ADP-dependent hexokinase (ADP-HK), 1000-80000U of AG-6-phosphate dehydrogenase and 0.1-0.3 mmol of adenosine diphosphate (ADP). The invention is used for determining 1,5- anhydro-D-glucitol.

Description

Technical field:

[0001] The invention relates to a reagent for the determination of 1,5-anhydrosorbitol by an enzymatic method. Background technique:

[0002] Diabetes is a common and frequently-occurring disease in clinical practice. It is caused by insufficient insulin secretion. The level of AG in plasma is significantly negatively correlated with plasma glucose, HbAlc and fructosamine (FMN). According to data, there is no case of plasma glucose > 8.3mmol / L. Associated with high levels of plasma AG

[12] . The plasma AG of diabetic patients is significantly lower than that of healthy people, especially in type 1 diabetic patients compared with type 2 diabetic patients. The plasma AG of new-onset and long-term patients with type 1 diabetes is generally below 1umol / L

[13] , and even reduced to undetectable out. Determination of 1,5-sorbitol has high performance liquid chromatography, micro-column chromatography, chemiluminescence and so on. These methods are relatively ac...