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Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium

A biocontrol yeast and disease control technology, which is applied in the field of fruit postharvest disease prevention and control, can solve the problems of no induction to improve the biocontrol yeast medium, achieve outstanding disease control effects, enhance the secretion of antibacterial substances, and inhibit the occurrence and development of Effect

Inactive Publication Date: 2010-10-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is currently no medium specifically used to induce and improve the effectiveness of biocontrol yeasts on fruit disease control

Method used

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  • Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium
  • Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium
  • Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] First, the configuration of the culture medium:

[0054] NYCA activation medium: 8g of beef extract, 5g of yeast powder, 10g of glucose, 20g of agar, 5g of chitin, and water to a volume of 1000ml, and autoclave (121°C, 30 minutes) to obtain NYCA activation medium;

[0055] NYCB seed culture medium: 8 g of beef extract, 5 g of yeast powder, 10 g of glucose, 5 g of chitin, and water to a volume of 1000 ml were autoclaved (121°C, 30 minutes) to obtain NYCB seed culture medium.

[0056] 2. Activation and preparation of strains:

[0057] 1), activation:

[0058] ①. Select Cryptococcus laurentis CGMCC NO.3590 stored in NYCA activation medium at low temperature (4°C);

[0059] The above-mentioned Cryptococcus laurentii CGMCC NO.3590 was cultured in NYCA activation medium at 28°C for 48 hours, and then subcultured twice under the same conditions (each time was cultured in NYCA activation medium at 28°C for 48 hours). ).

[0060] ②. Transfer the activated yeast obtained from...

Embodiment 2

[0068] Embodiment 2, the weight of chitin in the NYCB seed culture medium is changed from 5g to 10g, all the other are the same as embodiment 1.

Embodiment 3

[0069] Embodiment 3, the weight of chitin in the NYCB seed culture medium is changed from 5g to 20g, all the other are the same as embodiment 1.

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Abstract

The invention discloses a culture medium for improving prevention and control effects of biological control yeast on fruit diseases by induction, which comprises an activation culture medium and a seed culture medium, wherein the activation culture medium is prepared into NYCA activation culture medium from 8 grams of beef extract, 5 grams of yeast powder, 2 to 30 grams of glucose, 20 grams of agar and 1 to 50 grams of chitin by fixing volume to 1,000 milliliters with water and sterilizing the mixture under high pressure; and the seed culture medium is prepared into NYCB seed culture medium from 8 grams of beef extract, 5 grams of yeast powder, 2 to 30 grams of glucose and 1 to 50 grams of chitin by fixing volume to 1,000 milliliters with water and sterilizing the mixture under high pressure. The invention also discloses a method for improving the prevention and control effects of the biological control yeast on the fruit diseases by induction by using the culture medium at the same time. The method comprises steps of performing activation and liquid culture and the like on cryptococcus laurentii CGMCC No.3590.

Description

technical field [0001] The invention relates to the technical field of fruit post-harvest disease prevention and control. Background technique [0002] Traditionally, the control of fruit postharvest diseases at home and abroad mainly relies on fungicides. However, with the continuous enhancement of people's awareness of food safety and environmental protection, the scope and dosage of chemical fungicides are increasingly restricted. In developed countries in Europe and the United States, many chemical fungicides that are highly effective but have hidden dangers of food safety and environmental pollution are banned from use. Therefore, the research and development of methods and technologies that can replace chemical fungicides have great social significance and broad application prospects. [0003] Among the many researched methods that may replace chemical fungicides, the biological control technology of using antagonistic microorganisms, especially antagonistic yeasts t...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/645
Inventor 余挺郑晓冬卢黄娉王一非
Owner ZHEJIANG UNIV
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