Chinese herb medicinal additive for preventing tilapia fatty liver disease

A Chinese herbal medicine additive, fatty liver disease technology, applied in application, animal feed, animal feed, etc., can solve problems such as increased muscle fat content, slow growth of tilapia, and decreased anti-stress ability, so as to prevent nutritional fatty liver disease , Improving disease resistance and stabilizing the source

Active Publication Date: 2010-11-24

GUANGDONG OCEAN UNIVERSITY

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AI-Extracted Technical Summary

Problems solved by technology

Nutritional fatty liver disease can lead to slow growth of tilapia, decreased anti-stress ability, and a large number of death of tilapia in high temperature seasons; commercial fish suffering from fatty ...

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Method used

Result of the test shows: after adding this compound Chinese herbal medicine additive of 1.5% in commodity tilapia feed, the weight gain rate of test group tilapia, survival rate, plumpness, improve 30.22%, 9.88% respectively than matched group %, 5.45%, and the feed coefficient decreased by 22.67%; the biochemical indicators such as the significant alanine aminotransferase activity, aspartate aminotransferase activity, total cholesterol content, and triglyceride content in the serum of tilapia were significantly lower than those of the control group; the liver body of tilapia Compared with the control group, the fat content in...

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Abstract

The invention relates to a Chinese herb medicinal additive for preventing tilapia fatty liver disease. The Chinese herb medicinal additive is characterized by comprising the following components in part by weight: 8 to 10 parts of mung bean, 1 to 3 parts of liquoric root, 1 to 3 parts of gentian, 1 to 3 parts of raw gardenia, 1 to 3 parts of baical skullcap root, 1 to 3 parts of bupleurum, 2 to 5 parts of rehmannia root, 1 to 3 parts of plantain herb, 1 to 3 parts of virgate wormwood herb, 1 to 3 parts of indigowoad root, 1 to 3 parts of Mongolian dandelion herb, 2 to 5 parts of feverfew, 1 to 3 parts of turmeric root-tuber and 1 to 3 parts of oriental waterplantain rhizome. The Chinese herb medicinal additive has the advantages of capacities of obviously reducing liver weight/body weight and fat content of muscle and liver of the tilapia, reducing glutamic-oxalacetic transaminase activity, glutamic-pyruvic transaminase activity, total cholesterol content and triglycercide content in tilapia serum, protecting liver cells from the damage of factors such as nutritional factors, environmental factors and the like and effectively preventing nutritional fatty liver disease in the breeding process of the tilapia, convenient addition and use, capacity of being added in a commercial feed directly, synergistic effect of the components and other additive components in the commercial feed, improvement on the disease resistance of the tilapia, stable raw material source, low cost, simple production process and easy industrialized production.

Application Domain

Animal feeding stuff

Technology Topic

Glutamic-Pyruvic TransaminaseDandelion +22

Examples

Experimental program(1)

Example Embodiment

[0011] Implementation example 1

[0012] (1) After crushing 14 Chinese herbal medicines such as mung bean, licorice, gentian, raw gardenia, scutellaria, and Bupleurum, respectively, through a 200-mesh sieve, then press 8-10 parts of mung beans; 1-3 parts of licorice; gentian 1-3 parts; Raw Gardenia 1-3 parts; Scutellaria baicalensis 1-3 parts; Bupleurum 1-3 parts; Raw land 2-5 parts; Plantain 1-3 parts; Yinchen 1-3 parts; Banlangen 1- 3 parts; 1-3 parts of dandelion; white chrysanthemum: 2-5 parts; 1-3 parts of turmeric and 1-3 parts of Alisma and mixing evenly (requirement coefficient of variation ≤5%) is the compound Chinese herbal medicine of the present invention additive.

[0013] (2) Add the prepared compound Chinese herbal medicine additives to the commercial tilapia feed at the proportions of 0.5%, 1.0%, 1.5%, and 2.0% (weight percentage) respectively, and mix them to prepare 4 kinds of granular medicines. bait.

[0014] (3) Feed tilapia with the prepared bait in (1) (Tilapia farming was carried out in the High-tech Park of Guangdong Ocean University, Donghai Island Experimental Zone, Zhanjiang City. The experimental group and the control group were each set up with three replicates, and the control The group fed commercial tilapia feed), and carried out feeding management according to scientific feeding methods. Oxygen is supplied by the oxygen dispenser and the water is changed once every two days. The daily feeding amount is 5-10% of the fish's body weight, and the feeding is divided into two feedings (9 am and 6 pm), and the feeding period is 56 days.

[0015] (4) On the 57th day, after weighing and counting the fish in the test group and the control group, 15 tilapia fishes were randomly selected from each group to collect blood from the tail vein, and then liver tissues were taken under aseptic conditions. Measure the weight gain rate, survival rate, liver-to-body ratio, fullness, fat content in muscle and liver and other growth performance indicators of tilapia; determine serum alanine aminotransferase activity, aspartate aminotransferase activity, total cholesterol content, triglyceride Biochemical indicators such as lipid content, and analyze liver tissue sections.

[0016] (5) On the 57th day, 15 tilapias were randomly selected from each group, and 1×10 8 The cells/ml of Aeromonas hydrophila were challenged by intramuscular injection at a dose of 0.1ml/tail. The death of tilapia in each group within 10 days was observed and recorded, and the immune protection rate was calculated.

[0017] The test results showed that after adding 1.5% of this compound Chinese herbal medicine additive to commercial tilapia feed, the weight gain, survival rate, and fullness of tilapia in the test group were increased by 30.22%, 9.88%, and 5.45 respectively compared with the control group. %, feed coefficient decreased by 22.67%; significant alanine aminotransferase activity, aspartate aminotransferase activity, total cholesterol content, triglyceride content and other biochemical indicators in tilapia serum were significantly lower than those of the control group; tilapia liver-to-body ratio, muscle And the fat content in the liver was also significantly lower than that in the control group; liver tissue slices showed that the blood vessel network in the experimental group was clear, the distribution was normal, and the cell membrane and nucleus of liver cells were clear, which was the same as the results of liver tissue slices of healthy tilapia. In the control group during the same period, focal necrosis occurred, the nuclear membrane and nucleolus of liver cells could not be identified, and some liver cells had swollen and died; after artificial infection with Aeromonas hydrophila, the immune protection rate of tilapia was 69.1 %.

[0018] The above implementation examples show that the present invention can not only effectively prevent the occurrence of nutritional fatty liver disease in the tilapia breeding process, but also can significantly improve the growth performance index and disease resistance of tilapia.

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