Hepatitis B virus dendritic cell therapeutic vaccine and preparation method thereof

A technology of dendritic cells and therapeutic vaccines, applied in the field of therapeutic vaccines and their preparation, can solve the problems of insufficient specific cellular immune activation, peripheral immune tolerance, and low immune response, and achieve efficient induction of antiviral replication, The effect of promoting T cell immune response

Inactive Publication Date: 2010-12-01
ARMY MEDICAL UNIV
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Problems solved by technology

[0004] However, chronic HBV patients have problems such as low immune response, insufficient activation of specific cellular immunity, and peripheral immune tolerance. When designing therapeutic vaccines, it is necessary to enhance the ability of the immune system to recognize HBV to stimulate powerful specific cellular immunity.

Method used

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  • Hepatitis B virus dendritic cell therapeutic vaccine and preparation method thereof
  • Hepatitis B virus dendritic cell therapeutic vaccine and preparation method thereof
  • Hepatitis B virus dendritic cell therapeutic vaccine and preparation method thereof

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Embodiment Construction

[0031] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0032] 1. Preparation of Dendritic Cell Therapeutic Vaccine of Hepatitis B Virus

[0033] 1. Construction of recombinant eukaryotic vector for co-expression of HBcAg and Fc genes

[0034] (1) Cloning of HBcAg gene

[0035] According to the HBcAg gene sequence with GenBank accession number NC_003977.1, the following primers were designed and synthesized to amplify the full-length HBcAg cDNA with restriction sites at both ends: F1: 5’-aat ctgcag ggcatggacatcgacccttat-3' (SEQ ID No: 3), the under...

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Abstract

The invention discloses a hepatitis B virus dendritic cell therapeutic vaccine and a preparation method thereof. The preparation method thereof includes the steps that: HBcAg-Fc digenic co-expression recombinant eukaryotic vector is constructed; the obtained digenic co-expression recombinant eukaryotic vector is added with saponin Quil A and then is added with cholesterol and lecithin, ice-bath ultrasonic processing is carried out, so that solution is fully mixed and emulsified, PBS dialysis is carried out, and the obtained micro floccus is immunostimulating complex; and the obtained immunostimulating complex activates and simulates dendritic cell, thus obtaining the cell therapeutic vaccine. The vaccine carries out high efficiency transfection on dendritic cell, the generated fusion protein targets the dendritic cell in high efficiency by virtue of Fc segment, thus activating the dendritic cell, promoting immune response of hepatitis B virus core antigen specificity T cell and inducing the antivirus replication capability of immune cell in high efficiency, so that the vaccine can be taken as hepatitis B virus therapeutic vaccine.

Description

technical field [0001] The invention relates to a therapeutic vaccine and a preparation method thereof, in particular to a preparation method of a hepatitis B virus dendritic cell therapeutic vaccine and the prepared hepatitis B virus dendritic cell therapeutic vaccine. Background technique [0002] Hepatitis B virus (HBV) is a hepatotropic DNA virus. Hepatitis B virus hepatitis B, referred to as hepatitis B, is caused by HBV, mainly through blood, body fluids and mother-to-child transmission, and has a chronic carrier state of infectious diseases. At present, there are more than 300 million chronic HBV infections in the world, about half of which are in my country. This kind of infection is easy to develop into chronic hepatitis and cirrhosis, and a small number of cases can be transformed into primary hepatocellular carcinoma. It is currently the ninth cause of death among human diseases announced by WHO. Therefore, seeking an ideal anti-HBV drug has always been an urgen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/29A61P31/20
Inventor 吴玉章杨曌
Owner ARMY MEDICAL UNIV
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