Hepatitis B virus dendritic cell therapeutic vaccine and preparation method thereof

A technology of dendritic cells and therapeutic vaccines, applied in the field of therapeutic vaccines and their preparation, can solve the problems of insufficient specific cellular immune activation, peripheral immune tolerance, and low immune response, and achieve efficient induction of antiviral replication, The effect of promoting T cell immune response
CN101897965AInactive Publication Date: 2010-12-01ARMY MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
ARMY MEDICAL UNIV
Publication Date
2010-12-01
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a hepatitis B virus dendritic cell therapeutic vaccine and a preparation method thereof. The preparation method thereof includes the steps that: HBcAg-Fc digenic co-expression recombinant eukaryotic vector is constructed; the obtained digenic co-expression recombinant eukaryotic vector is added with saponin Quil A and then is added with cholesterol and lecithin, ice-bath ultrasonic processing is carried out, so that solution is fully mixed and emulsified, PBS dialysis is carried out, and the obtained micro floccus is immunostimulating complex; and the obtained immunostimulating complex activates and simulates dendritic cell, thus obtaining the cell therapeutic vaccine. The vaccine carries out high efficiency transfection on dendritic cell, the generated fusion protein targets the dendritic cell in high efficiency by virtue of Fc segment, thus activating the dendritic cell, promoting immune response of hepatitis B virus core antigen specificity T cell and inducing the antivirus replication capability of immune cell in high efficiency, so that the vaccine can be taken as hepatitis B virus therapeutic vaccine.
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Description

technical field

[0001] The invention relates to a therapeutic vaccine and a preparation method thereof, in particular to a preparation method of a hepatitis B virus dendritic cell therapeutic vaccine and the prepared hepatitis B virus dendritic cell therapeutic vaccine. Background technique

[0002] Hepatitis B virus (HBV) is a hepatotropic DNA virus. Hepatitis B virus hepatitis B, referred to as hepatitis B, is caused by HBV, mainly through blood, body fluids and mother-to-child transmission, and has a chronic carrier state of infectious diseases. At present, there are more than 300 million chronic HBV infections in the world, about half of which are in my country. This kind of infection is easy to develop into chronic hepatitis and cirrhosis, and a small number of cases can be transformed into primary hepatocellular carcinoma. It is currently the ninth cause of death among human diseases announced by WHO. Therefore, seeking an ideal anti-HBV drug has always been an urgen...

Claims

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