Internal circulation gas-lift type cell culture apparatus

Abstract

The invention relates to an internal circulation gas-lift type cell culture apparatus which is mainly suitable for cell culture of tissues in agriculture and forestry. The invention is characterized in that a cell culture bottle is provided with an air disperser, a culture bottle bleeder hole and a culture bottle air inlet, wherein the air disperser and the culture bottle air inlet are positionedat the middle lower part of the bottle body, and the culture bottle bleeder hole is positioned at the middle upper part of the bottle body; an internal gas circulation mechanism is arranged in the cell culture apparatus and comprises a transmission fixed seat, a wearproof rope, a pair of piston bodies which are installed in opposite directions, a pair of return springs and a pair of return springclamps; the internal gas circulation mechanism is connected with the cell culture bottle; and each piston body is provided with a piston air inlet and a piston air outlet. The invention has reasonable structural design, and gas in the cell reactor is circulated into the culture fluid through a minitype one-way piston pump, so that the cells of the plant tissues in the cell culture bottle are in the gas-lift culture state; and the apparatus is easy to disassemble and assemble, can not be affected by damage and replacement, and has the advantages of low cost and easy popularization.

Description

technical field [0001] The invention relates to a cell culture device, in particular to an internal circulation air-lift cell culture device, which is mainly suitable for cell culture of agricultural and forestry organizations, especially for those sensitive to shear force and external gas to obtain important secondary growth Cultivation of medicinal plant cells for metabolite purposes. Background technique [0002] At present, there are many devices for culturing plant tissue cells, and plant cells can synthesize many valuable secondary metabolites, including medicinal active substances. The use of large-scale suspension culture of plant cells in bioreactors to produce useful metabolites of plants has made great progress in recent years. The design and development of reactors mainly focus on the design of bioreactors with high mixing efficiency and low shear strength. and making. [0003] At present, reactors suitable for plant tissues and cells mainly include mechanicall...

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Problems solved by technology

[0010] The technical problem to be solved by the present invention is to overcome the complex equipment caused by the use of external air source in the prior art, and to drive away the defects of carbon dioxide and ethylene in the cell fluid after aeration, and to provide a reasonable structural design, which can cultivate Internal circulation air-lift cell culture device for medicinal plant cells

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