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Bombyx mori nuclear polyhydrosis virus (BmNPV) 39k inducible promoter and application thereof

A karyotype polyhedron, inducible technology, applied in the direction of using vectors to introduce foreign genetic material, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems that have not yet been reported on the BmNPV inducible promoter, and achieve inhibition of virus proliferation. Effect

Inactive Publication Date: 2012-07-04
SOUTHWEST UNIV
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Problems solved by technology

But so far, there is no research report on the BmNPV-inducible promoter

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  • Bombyx mori nuclear polyhydrosis virus (BmNPV) 39k inducible promoter and application thereof
  • Bombyx mori nuclear polyhydrosis virus (BmNPV) 39k inducible promoter and application thereof
  • Bombyx mori nuclear polyhydrosis virus (BmNPV) 39k inducible promoter and application thereof

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Embodiment Construction

[0020] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0021] 1. Selection and cloning of BmNPV-inducible promoter

[0022] Download the complete genome sequence of BmNPV (NC001962.1) from the National Center for Biotechnology Information (NCBI) in the United States. According to the cascade regulation characteristics of baculovirus, the delayed early gene promoter (β gene) is controlled by the product of the immediate early gene (α gene) To activate the regulatory function, the 39k promoter of the delayed early gene of BmNPV was selected as a candid...

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Abstract

The invention discloses a bombyx mori nuclear polyhydrosis virus (BmNPV) 39k inducible promoter and application thereof. A nucleotide sequence of the promoter is shown as SEQ ID No.1; the promoter has obvious BmNPV inducible promotion activity and can make cells start foreign gene expression when the cells are subjected to the BmNPV infection; an RNAi vector using a BmNPV multiplication essential gene as a target can be built by utilizing the promoter; the abundant expression of shRNA can be performed when the bombyx mori is subjected to the BmNPV infection; the shRNA is cut into siRNA in a cell by an enzyme; specific degradation is performed on the BmNPV multiplication essential gene mRNA to initiate the BmNPV multiplication essential gene to be transcribed and then silenced, so the aim of inhibiting the virus multiplication is fulfilled, and the promoter can be used for preventing and controlling the BmNPV infection of the bombyx mori, performing genetic engineering breeding of bombyx mori anti-BmNPV strains and providing a good reference mode for transgenic therapy of other biologic virus diseases.

Description

technical field [0001] The invention relates to the biological field, in particular to a Bombyx mori nuclear polyhedrosis virus (BmNPV) 39k inducible promoter and an application thereof. Background technique [0002] The silkworm is a Lepidoptera insect with important economic value. BmNPV is the most common and most harmful type of pathogen in sericulture production, which has a huge impact on sericulture. How to fundamentally improve the silkworm's virus resistance by using the rapidly developing genetic engineering technology is an important research topic in the field of sericulture. Gene knockout studies have shown that BmNPV will not be able to proliferate without certain genes essential for proliferation. Therefore, inhibiting the expression of these genes essential for virus proliferation will be an effective strategy to prevent and control BmNPV infection in silkworms. [0003] RNA interference (RNA interference, RNAi) refers to the phenomenon of gene post-transc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01K67/04
Inventor 潘敏慧鲁成李娜赵爱春夏庆友向仲怀
Owner SOUTHWEST UNIV
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