Method for extracting orientin from bamboo leaf

A technology of orientin and bamboo leaves, which is applied in the field of natural product separation, to achieve the effects of enhanced separation effect, good separation effect and short cycle

Inactive Publication Date: 2011-01-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing literature, there are few studies on the extraction and separation of orientin from bamboo leaves.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 2kg of bamboo leaf crushed material, add 18L lime water (control the pH at 8-9) and 30g borax, mix well, heat to 75℃ for 3 hours, filter, and extract the residue again according to the above conditions, combine the filtrate and the filtrate Add 8% hydrochloric acid solution to adjust the pH to 5, pass the 200 mesh polyamide chromatographic column adsorption, elution with 3BV 65% ethanol solution, concentrate the eluate, and then extract with n-butanol 3 times, evaporate Butanol, mix well with 40g ODS silica gel with a particle size of 50μm, dry it, dry it on a silica gel column, use ethanol-water-acetic acid mixed solution gradient elution, pressure control flow rate 30ml / min, thin layer monitoring, collect The oxaloside fraction was concentrated to recover ethanol, and vacuum dried to obtain 6.8 g of product with a content of 97.1%.

Embodiment 2

[0028] Take 2kg of bamboo leaf crushed material, add 20L lime water (control the pH at 8-9) and 25g borax, mix well, heat to 70℃ for 3 hours, filter, and extract the filter residue again according to the above conditions. Combine the filtrate and the filtrate Add 5% hydrochloric acid solution to adjust the pH to 4.5, pass the 200 mesh polyamide chromatographic column adsorption, elution with 2BV 70% ethanol solution, concentrate the eluate, and then extract twice with n-butanol, evaporate and dry Butanol, mix well with 35g ODS silica gel with a particle size of 50μm, dry, apply dry method to silica gel column, elution with ethanol-water-acetic acid solution gradient, pressure control flow rate 50ml / min, thin layer monitoring, collect Orientia The glycoside fraction was concentrated to recover ethanol, and vacuum dried to obtain 7.4 g of product with a content of 97.5%.

Embodiment 3

[0030] Take 5kg of bamboo leaf crushed material, add 60L lime water (control pH 8-9) and 60g borax, mix well, heat to 65℃ for 3 hours, filter, add 50L lime water (control pH 8-9) and 50g the filter residue After the borax is evenly mixed, heat to 60°C for extraction for 2 hours, filter, combine the filtrate, add 8% hydrochloric acid solution to the filtrate to adjust the pH to 5, pass through a 300 mesh polyamide chromatography column for adsorption, use 2.5BV of 75% ethanol The solution is eluted, the eluate is concentrated, and then extracted 3 times with n-butanol. The n-butanol is evaporated and mixed with 70g of ODS silica gel with a particle size of 50μm, dried, and dried on a silica gel column with ethanol-water- Gradient elution of acetic acid mixed solution, pressure control flow rate 40ml / min, thin layer monitoring, collect orientin fractions, concentrate to recover ethanol, vacuum dry to obtain product 16.2g, content 95.3%.

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Abstract

The invention provides a method for providing active compound orientin with myocardium nourishing function from bamboo leaf. The method is characterized in that bamboo leaf raw material is smashed, the smashed bamboo leaf is added into 8-12 times alkaline water containing borax, heating extraction is carried out for 2-3 times, extracting solution is acidized and then is added into a polyamide chromatographic column, ethanol-water mixed solvent is used for elution, eluent is concentrated, butyl alcohol is used for extraction for 2-3 times, an ODS silicagel column is used for separation, methyl alcohol-water-acetic acid mixed solvent is used for pressurization and elution, thin-layer chromatography monitoring is carried out, orientin fraction is collected, and vacuum drying is carried out, thus obtaining the product. The method has simple operation, high product content and good repeatability.

Description

Technical field: [0001] The invention belongs to the field of natural product separation, and particularly relates to a method for extracting orientin from bamboo leaves. Background technique: [0002] Orientin, a flavonoid glycoside compound, is easily soluble in water and ethanol, but hardly soluble in organic solvents such as benzene, chloroform, and ether. [0003] Orientin is the main component of bamboo leaf flavonoids. Flavonoids have obvious biological and physiological activities such as antiulcer, antispasmodic, antibacterial, anti-inflammatory, hypolipidemic, analgesic, and estrogen. [0004] Bamboo leaves have a long history of medicinal use in our country, and they are blindly famous for clearing away heat and detoxification. The functional factors of bamboo leaf extract are mainly flavonoid glycosides. Bamboo leaf flavonoids have various biological activities, such as anti-lipid peroxidation, anti-aging, scavenging free radicals, blocking nitrosation, reducing blood li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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