Tissue culture breeding method for metabriggsia ovalifolia

A technology of tissue culture and single-seat lettuce, which is applied in the field of plant tissue culture, can solve problems that have not been reported, and achieve the effect of accelerating the reproduction speed

Inactive Publication Date: 2011-02-09
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But, up to now, the research on the tissue culture aspect of Lettuce uniflora, all have not been reported at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take the leaves of a single-seat Lettuce plant collected from Hechi, Guangxi as explants, clean the leaf explants with tap water, scrub and disinfect the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and wash off with sterile water 2 times, then soaked in 0.1% mercuric chloride solution by mass volume percentage for 8 minutes, washed 3 times with sterile water, and finally cut the leaves into 0.6cm 2 Size, inoculated on the induction medium and cultivated, the culture condition is 22~28°C, cultivated in the dark, induced adventitious buds after 40 days, and then transferred to a light intensity of 50μmol m -2 the s -1 , Continue to cultivate for 20 days under the condition of 14 hours / day of light; then transfer the adventitious buds to the propagation medium to proliferate the adventitious buds, the culture conditions are 22~28°C, and the light intensity is 50 μmol m -2 the s -1 , light 14 hours / day, one month is a subculture cycle,...

Embodiment 2

[0027] Take the leaves of a single-seat Lettuce plant collected from Hechi, Guangxi as explants, clean the leaf explants with tap water, scrub and disinfect the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and wash off with sterile water 2 times, then soaked in 0.1% mercuric chloride solution by mass volume percentage for 8 minutes, washed 3 times with sterile water, and finally cut the leaves into 0.6cm 2 Size, inoculated on the induction medium and cultured at 22-28°C, with a light intensity of 20 μmol m -2 the s -1 , light 14 hours / day, induce adventitious buds after 40 days, and then switch to a light intensity of 80μmol m -2 the s -1 1. Continue culturing for 10 days under the condition of 14 hours / day of light; then transfer the adventitious buds to the propagation medium to proliferate the adventitious buds. -2 the s -1 , light 14 hours / day, one month is a subculture cycle, and each subculture cycle proliferates 4.6 times. The p...

Embodiment 3

[0030] Take the leaves of a single-seat Lettuce plant collected from Hechi, Guangxi as explants, clean the leaf explants with tap water, scrub and disinfect the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and wash off with sterile water 2 times, then soaked in 0.1% mercuric chloride solution by mass volume percentage for 8 minutes, washed 3 times with sterile water, and finally cut the leaves into 0.6cm 2 size, inoculated on the induction medium and cultured at 22-28°C, with a light intensity of 10 μmol m -2 the s -1 , light 14 hours / day, induce adventitious buds after 40 days, and then switch to a light intensity of 60μmol m -2 the s -1 , Continue culturing for 15 days under the condition of 14 hours / day of light; then transfer the adventitious buds to the propagation medium for the proliferation of adventitious buds, the culture conditions are 22-28°C, and the light intensity is 60 μmol m -2 the s -1 , light 14 hours / day, one month i...

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PUM

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Abstract

The invention discloses a tissue culture breeding method for metabriggsia ovalifolia. In the method, the totipotency of plant cells and plant tissue culture technology are utilized; leaf blades of the metabriggsia ovalifolia are taken as explants and are cultured in a proper culture medium under proper culture conditions after sterilizing, so that the leaf blades can dedifferentiate and differentiate to form adventitious buds; test tube plantlets are obtained by subculture multiplication and rooting culture; and planting seedlings are obtained by transplanting, cultivating and hardening the test tube plantlets. In the method, the planting seedlings can be obtained in 140 days from the induction of the explants, which greatly accelerates the breeding speed of the metabriggsia ovalifolia; through the subculture multiplication, the test tube seedlings can be multiplied by about 4.6 times, so a large number of test tube plants can be obtained in a short time; and after transplanting, the survival rate of the test tube plantlets is up to over 90 percent, so that the metabriggsia ovalifolia of the threatened plants at a national level is preserved and developed, and a good foundation is laid for better utilizing the species in future.

Description

Technical field: [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and propagation of a single lettuce. Background technique: [0002] Metabriggsia ovalifolia (Metabriggsia ovalifolia W.T.Wang) belongs to the family Gesneriaceae (Gesneriaceae), a small perennial herbaceous plant with a stem height of 20-40 cm and covered with brown villous hairs. It is currently distributed in Napo and Nandan, Guangxi, China, and grows in the forests of limestone hills at an altitude of 1100 meters. Due to the special living environment requirements, narrow geographical distribution and small population of this species, it has been identified by the state as the first batch of rare and endangered plants under key (level one) protection in China. [0003] Using plant explants to cultivate on solid medium to obtain callus or complete plants is plant tissue culture, also known as in vitro culture or explant culture. Sin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马国华张新华赵杰堂杨兴玉吕金凤胡玉姬
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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