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Culture method for effectively improving total alkaloid content of Fritillaria cirrhosa D. Don

A technology of fritillary fritillary and total alkaloids, which is applied in the direction of plant cells, etc., to achieve the effects of solving the long growth cycle, increasing the biomass of the culture, and increasing the total alkaloid content

Active Publication Date: 2012-08-29
薛刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the biotechnology research of Fritillaria rollata, there is no report on the use of precursor feeding technology to significantly increase the total alkaloid content of Fritillaria rollosa in liquid culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Acquisition of regenerated bulbs of Fritillaria fritillata: Cut the induced compact callus that has begun to differentiate into small pieces of 1.5 cm, and transfer to the medium MS+6-BA1mg L for producing bulbs -1 +NAA0.2mg·L -1 + sucrose 40g·L -1 +Agar 6.5g·L -1 Cultivation in the medium, the pH value used is 6.0, the cultivation temperature is 15-23°C, the light is 8-16 hours a day, and the light intensity is 1000-1800lx; after about 40 days of cultivation, a large number of well-growing regenerated bulbs can be obtained;

[0015] (2) Liquid culture of the regenerated bulbs of Fritillaria convolute: Cut the ungerminated young regenerated bulbs of Fritillaria convolute with a diameter of 3-6 mm obtained in step (1), and transfer them to the liquid medium MS added with tryptophan +6-BA 2.0mg·L -1 +NAA 0.4mg·L -1 + sucrose 30g·L -1 Cultured in , the pH value was 6.5, and the inoculum size was 50g·L -1 , the culture temperature is 15-23°C, the light is 8-16 hou...

Embodiment 2

[0018] The amino acid precursor added in the step (2) of this embodiment is tryptophan, and the addition amount is 1.00mmol L -1 , the other steps are the same as in Example 1, and the total alkaloid content measured in the culture after 25 days of cultivation is 0.140%, which is significantly lower than the total alkaloid content of 0.212% in the regenerated bulb of Fritillaria vol. There is an optimal concentration for adding amino acid precursors in Fritillaria japonicus liquid medium, adding different concentrations of precursors has a significant effect on the total alkaloid content of the regenerated bulbs of Fritillaria rollifolius.

Embodiment 3

[0020] The amino acid precursor added in the step (2) of this embodiment is phenylalanine, and the addition amount is 0.25mmol L -1 , the other steps were the same as in Example 1. After 25 days of cultivation, the total alkaloid content in the culture was measured to be 0.173%, which was 22.4% higher than the total alkaloid content of the control group without adding any amino acid precursor (0.139%).

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Abstract

The invention discloses a culture method for effectively improving the total alkaloid content of Fritillaria cirrhosa D. Don, which is characterized in that: in the method, precursor-feeding technology is adopted, and in the liquid culture process of young regenerated bulbs of Fritillaria cirrhosa D. Don, the total alkaloid content of Fritillaria cirrhosa D. Don cultures is improved by adding amino acid precursors. The experimental results show that the total alkaloid content of final obtained Fritillaria cirrhosa D. Don cultures can reach 0.212 percent after 25-day culturing, which is 52.5 percent higher than that of the control group without adding amino acid precursors. The invention not only effectively improves the total alkaloid content of Fritillaria cirrhosa D. Don in liquid culture, but also significantly increases the reproductive rate of biomass of Fritillaria cirrhosa D. Don, thereby achieving the purpose of rapidly producing the effective ingredients of unibract fritillary bulbs better.

Description

technical field [0001] The invention relates to a cultivation method for increasing the total alkaloid content of Fritillaria curica, in particular to a cultivation method for effectively increasing the total alkaloid content of Fritillaria convolute by adding specific amino acid precursors in a liquid medium. Background technique [0002] Fritillaria cirrhosa D.Don is a perennial herbaceous plant of Liliaceae, and its bulb is the precious Sichuan medicinal material Fritillaria cirrhosa. The Chinese herbal medicine Chuan Fritillaria is used to treat lung-heat dry cough, yin-deficiency labor cough, lung-deficiency chronic cough and other diseases. The effect is remarkable, and the annual dosage is increasing year by year. There are more than 130 kinds of Chinese patent medicines of Fritillaria. For a long time, the medicinal materials of Fritillaria sichuanensis mainly depended on excavating wild medicinal materials. Due to factors such as excessive collection and habitat de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 王跃华朱丹青代勇孙燕霞何玉红邓启花徐世军王晓蓉陈然张阔军
Owner 薛刚
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