Quality detection method of asiatic toddalia root gargle

Abstract

The invention discloses a quality detection method of asiatic toddalia root gargle, comprising the following steps of: (1) identifying the asiatic toddalia root gargle; and (2) measuring the content according to a high-efficiency liquid chromatography: A, performing of a chromatograph condition and system adaptability test; B, comparison product solution preparation: precisely weighting a proper amount of isoferulic acid comparison product and preparing into the solution containing 20 mug per 1ml by using 10% ethanol; C, preparation of a test sample solution; and D, application of a measuringmethod: precisely absorbing 10 mul of the comparison production solution and 10 mul of the test sample solution, injecting in a liquid chromatograph for measuring, wherein every 100ml of the product contains at least 4.0mg of rhizoma cimicifugae metered by isoferulic acid. The invention has high test accuracy, can better control the quality of the medicine and ensure the medication safety, and the medicinal materials can be easily obtained.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicine preparations, in particular to a quality detection method for liquid in the mouth of a dragon's palm. Background technique [0002] Longzhangkou liquid is composed of the four medicinal materials of Feilongzhang blood root bark, Feilongzhang blood leaf, Digupi and Cimicifuga, add water and boil three times, the first time, the second time for 2 hours, the third time for 1 hour, and combine Decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.10-1.15 (60-70°C), add ethanol to make the alcohol content 70%, let it stand for 24 hours, take the supernatant, filter, and recover the filtrate Ethanol and concentrated to a clear paste with a relative density of 1.10-1.15 (60-70°C), add polysorbate 80, glycerin, mix well, boil, cool to below 40°C, add methylparaben, ethylparaben, Menthol, rose essence, coconut essence, add water to the spec...

AI Technical Summary

Problems solved by technology

The content determination in the original quality standard is to detect the ferulic acid contained in Cimicifuga in the prescription, but it is found in the long-term production that using ferulic acid as the content detection index has the following shortcomings for product quality control, 1. , A large number of documents have reported that the ferulic acid solution prepared with water or methanol (ethanol) is unstable, and it is easily decomposed when exposed to light. Ferulic acid can be reduced to 20% of the relative content 2 hours after exposure to light, thereby reducing the accuracy of the test. sex

2. Taking ferulic acid as the content measurement index, the transfer rate of the finished product is about 20%. In order to ensure that the finished product is qualified, the ferulic acid content of the medicinal materials should not be less than 0.07%, and most of the commercially available medicinal materials contain less than 0.07% ferulic acid. If this limit cannot be reached, it is not easy to purchase medicinal materials that meet the requirements

3. In liquid chromatography, the separation between the ferulic acid peak and other adjacent peaks is lower than 1.5, and the peak shape is poor and the column efficiency is low, thus affecting the accuracy of the test results

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