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Kit for determining mitochondria aspartate aminotransferase

A technology of aspartic acid amino group and aspartic acid, which is applied in the field of mitochondrial aspartate aminotransferase assay kit, can solve the problems of time-consuming determination, lack of severity of organ damage, difficulty in automatic determination, etc., and achieve The results are accurate

Inactive Publication Date: 2011-04-13
北京迪迈医学诊断技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Electrophoresis and chromatography are time-consuming, difficult to automate, and cannot be used for clinical large-scale specimens
Due to the above-mentioned methodological defects, the test of mitochondrial aspartate aminotransferase has not been widely used clinically for a long time, so the severity of damage to organs such as the liver and heart lacks direct indicators

Method used

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  • Kit for determining mitochondria aspartate aminotransferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Determination of Mitochondrial Aspartate Aminotransferase

[0020] Use the mitochondrial aspartate aminotransferase assay kit of the present invention to measure mitochondrial aspartate aminotransferase. The kit includes reagent 1 and reagent 2, and both reagent 1 and reagent 2 are liquid preparations. When measuring, the reagent 1 and 2 are used in combination.

[0021] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 1:

[0022] Table 1. Solutes in Reagent 1

[0023] Tris(Tris)

[0024] The solvent of reagent 2 is water, and the solute is as shown in Table 2:

[0025] Table 2. Solutes in Reagent 2

[0026] Tris

[0027] Samples to be tested: 7 samples of human non-hemolyzed serum or heparin anticoagulated plasma. The mitochondrial aspartate aminotransferase of 7 samples was determined by immunological method, and the results are shown in Table 3.

[0028] Table 3. Results of Immunological Assays for Mito...

Embodiment 2

[0042] Example 2 Mitochondrial Aspartate Aminotransferase Determination

[0043] Use the mitochondrial aspartate aminotransferase assay kit of the present invention to measure mitochondrial aspartate aminotransferase, the kit includes reagent 1 and reagent 2, and both reagent 1 and reagent 2 are liquid preparations, when measuring Reagents 1 and 2 are used in combination.

[0044] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 6:

[0045] Table 6. Solutes in Reagent 1

[0046] Tris

[0047] The solvent of reagent 2 is water, and its solute is as shown in Table 7:

[0048] Table 7. Solutes in Reagent 2

[0049] Tris

[0050] Samples to be tested: 7 human non-hemolyzed serum or heparin anticoagulated plasma samples in Example 1. The method for measuring mitochondrial aspartate aminotransferase activity is the same as the two-step method in Example 1. The results after measurement are shown in Table 8.

[0051] Table 8. The ...

Embodiment 3

[0054] Example 3 Determination of Mitochondrial Aspartate Aminotransferase

[0055] The measurement of mitochondrial aspartate aminotransferase uses the mitochondrial aspartate aminotransferase assay kit of the present invention, which kit includes reagent 1 and reagent 2, and reagent 1 and reagent 2 are liquid preparations, when measuring Reagents 1 and 2 are used in combination.

[0056] Wherein, the solvent of reagent 1 is water, and its solute is as shown in table 9:

[0057] Table 9. Solutes in Reagent 1

[0058] Tris

[0059] The solvent of reagent 2 is water, and the solute is as shown in Table 10:

[0060] Table 10. Solutes in Reagent 2

[0061] Tris

[0062] Samples to be tested: 7 human non-hemolyzed serum or heparin anticoagulated plasma samples in Example 1.

[0063] The method for measuring the activity of mitochondrial aspartate aminotransferase is the same as the two-step method in Example 1.

[0064] The results after measurement are sh...

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Abstract

The invention discloses a kit for determining mitochondria aspartate aminotransferase, containing a reagent 1 and a reagent 2, wherein the reagent 1 contains aspartic acid protease, alpha- ketoglutaric acid, L-aspartic acid, malate dehydrogenase, lactate dehydrogenase, a preservative and PEG6000. The enzyme activity ratio of the aspartic acid protease to the malate dehydrogenase to the lactate dehydrogenase in the reagent 1 is (0.1-50):(0.1-50):(0.1-10); the ratio of the alpha- ketoglutaric acid to the malate dehydrogenase is (0.1-60)g:(0.1-50)KU; the mass ratio of the alpha- ketoglutaric acid to the L-aspartic acid to the preservative to the PEG6000 is (0.1-60):(5-200):(0.2-10):(1-100); and the mass ratio of NADH (Reduced Form of Nicotinamide-Adenine Dinucleotid) to a preservative to EDTA (Ethylene Diamine Tetraacetic Acid).2Na is (0.5-10):(0.2-10):(1-50). The kit for determining the mitochondria aspartate aminotransferase is used for determining the activity of the determining mitochondria aspartate aminotransferase, is free from the interference of high cell plasma aspartate aminotransferase and obtains accurate result.

Description

technical field [0001] The invention relates to a kit for measuring mitochondrial aspartate aminotransferase. Background technique [0002] There are two isozymes of aspartate aminotransferase, mitochondrial aspartate aminotransferase and cytoplasmic aspartate aminotransferase. Mitochondrial aspartate aminotransferase and cytoplasmic aspartate aminotransferase were discovered by Hird and Rowsell in 1950 and confirmed by electrophoresis in 1960 by Fleisher et al. Experiments show that in alkaline solution, mitochondrial aspartate aminotransferase Acid aminotransferase moves towards the cathode, while cytoplasmic aspartate aminotransferase moves towards the anode. [0003] Mitochondrial aspartate aminotransferase and cytoplasmic aspartate aminotransferase are widely present in various tissue cells of the human body, and the liver and heart cells are the most abundant. Among them, the mitochondrial aspartate aminotransferase exists in the mitochondria, while the cytoplasmic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/52C12Q1/37C12Q1/32
Inventor 徐士峰杨玉军
Owner 北京迪迈医学诊断技术有限公司
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