34 results about "Aspartic Acid Proteases" patented technology

The invention discloses a kit for determining mitochondria aspartate aminotransferase, containing a reagent 1 and a reagent 2, wherein the reagent 1 contains aspartic acid protease, alpha- ketoglutaric acid, L-aspartic acid, malate dehydrogenase, lactate dehydrogenase, a preservative and PEG6000. The enzyme activity ratio of the aspartic acid protease to the malate dehydrogenase to the lactate dehydrogenase in the reagent 1 is (0.1-50):(0.1-50):(0.1-10); the ratio of the alpha- ketoglutaric acid to the malate dehydrogenase is (0.1-60)g:(0.1-50)KU; the mass ratio of the alpha- ketoglutaric acid to the L-aspartic acid to the preservative to the PEG6000 is (0.1-60):(5-200):(0.2-10):(1-100); and the mass ratio of NADH (Reduced Form of Nicotinamide-Adenine Dinucleotid) to a preservative to EDTA (Ethylene Diamine Tetraacetic Acid).2Na is (0.5-10):(0.2-10):(1-50). The kit for determining the mitochondria aspartate aminotransferase is used for determining the activity of the determining mitochondria aspartate aminotransferase, is free from the interference of high cell plasma aspartate aminotransferase and obtains accurate result.

The invention discloses a production method and applications of a novel rhizomucor miehei aspartic protease, and particularly relates to a recombinant aspartic protease, a coding gene, and a production method and applications of the recombinant aspartic protease. The recombinant aspartic protease is protein having an amino acid sequence shown as SEQ ID NO:1. The coding gene of the recombinant aspartic protease is a DNA molecule shown as SEQ ID NO:2. An aspartic protease gene that is RmproA of rhizomucor miehei is connected to a pichia pastoris GS115 expression vector pPIC9K, and is converted into pichia pastoris and induced expression is performed to obtain the recombinant aspartic protease. Through high-density fermentation in a fermentation tank having a volume of 5 L, the maximum protease production activity at 156 h of a recombinant strain is 3400 U/mL, and the protein content is 6.42 mg/mL. The recombinant aspartic protease is capable of effectively tendering pork, reducing shearing force, making meat palatable, and effectively hydrolyzing animal and plant protein to prepare low-molecular-weight polypeptides.

Described are compounds of the formula (I) which are orally active and bind to aspartic proteases to inhibit their activity. They are useful in the treatment or amelioration of diseases associated with aspartic protease activity. Also described are methods of use of the compounds described herein in ameliorating or treating aspartic protease related disorders in a subject in need thereof.

The invention discloses application of an aspartic protease gene in improvement of beauveria bassiana variety. The yield and toxicity of conidia of the beauveria bassiana are improved by knocking out the aspartic protease gene BbASP, or the high-temperature tolerance of the beauveria bassiana is improved by over-expressing the aspartic protease gene BbASP, and the nucleotide sequence of the aspartic protease gene BbASP is as shown in SEQ ID NO. 20; if the BbASP knockout strain is cultured for 10 d at 26 DEG C, the conidium yield is 72.57% higher than that of a wild type, if the BbASP knockout strain is cultured for 20 d at 26 DEG C, the conidium yield is 59.43% higher than that of a wild type, the survival rate of a host is reduced, the half lethal time is shortened, the time that entomogenous thalli penetrate out of the dead host is shortened, and the growth rate of the BbASP over-expression strain is increased under the high-temperature stress of 32 DEG C; and after the conidia are stressed at the high temperature of 43 DEG C for 2 h and 4 h, the germination rates are respectively increased by 7% and 11.34%.

Disclosed are compounds of Formula I, wherein the R, R¹, R^2, R³, X, Y, A, Q, E, and G are defined herein. These compounds bind to aspartic proteases to inhibit their activity and are useful in the treatment or amelioration of diseases associated with aspartic protease activity. Also disclosed are methods of use of the compounds of Formula I for ameliorating or treating aspartic protease related disorders in a subject in need thereof.

The invention discloses a method for assessing the heat resistance of a coral, and belongs to the field of ecological restoration of a marine environment. The method comprises the step that with phenotypic characteristics of the hermatypic coral, the symbiotic zooxanthellae density, the photosynthetic rate, superoxide dismutase (SOD), catalase (CAT), the aspartic acid protease (Caspase3) and the like as indexes, the heat resistance of the coral is assessed. The method has the advantages of multiple parameters, high precision, quantifiable comparison and the like, the method can be used for guiding coral transplanting and restoration, and a basis is provided for protection of a coral reef ecosystem. The method is easy and convenient to operate and easy to use, and the heat resistance of thecoral can be quickly assessed; by analyzing the phenotypic characteristics of the hermatypic coral, and the heat resistances of different kinds of corals can be compared so as to be used for seed selection in the coral breeding and coral transplanting and restoration processes; the heat resistances of the same kind of corals obtained before domestication and after domestication can be compared soas to used for assessing the domestication effect; moreover, the domestication effects of different kinds of corals can be compared so as to be used for seed selection during scientific research or coral transplanting and restoration.

The present invention describes an aspartic protease produced by a fungus from the class Eurotiomycetes, comprising the amino acid sequence of FDTGSSD or FDTGSSE. The present invention further provides a process for identifying new milk clotting enzymes comprising screening an amino acid sequence for the presence of FDTGSSD or FDTGSSE. The enzymes of the invention may he useful in cheese production.

The present invention is directed to aspartic protease inhibitors. The present invention is also directed to pharmaceutical compositions comprising the disclosed aspartic protease inhibitors. The present invention is further directed to methods of antagonizing one or more aspartic proteases in a subject in need thereof, and methods for treating an aspartic protease mediated disorder in a subject using the disclosed aspartic protease inhibitors.

The invention discloses a method for preparing a plant haploid by regulating an egg cell specific expression gene and application of the method in preparation of angiosperm haploid. The aspartic protease ecs1 ecs2 double mutant specifically expressed by the egg cells can be used for producing haplobionts for breeding. The method overcomes the influence of deletion of haploid induced genes expressed by a traditional male parent on pollen and fertility, provides a new thought for the biological action of aspartic protease in the haploid production process, and can improve the breeding efficiency of crop haploids.

The present invention is directed to aspartic protease inhibitors. The present invention is also directed to pharmaceutical compositions comprising the disclosed aspartic protease inhibitors. The present invention is further directed to methods of antagonizing one or more aspartic proteases in a subject in need thereof, and methods for treating an aspartic protease mediated disorder in a subject using the disclosed aspartic protease inhibitors.

Provided is a method for recombinant expression of β-glucosidase gene. Also provided is a recombinant expression vector comprising: (a) a coding sequence of an aspartic protease or active fragment thereof, (b) a coding sequence of β-glucosidase or active fragment thereof, and optionally (c) a linker sequence between (a) and (b). Further provided are the recombinant host cell and the recombinant cellulose-degrading microorganism comprising the recombinant expression vector, the preparation method and uses thereof.