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A heat-resistant, coral-based technology, applied in the direction of measuring devices, material analysis through optical means, instruments, etc., to achieve high precision, easy to use, and simple operation
Inactive Publication Date: 2019-11-29
GUANGXI UNIV
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[0006] The purpose of the present invention is to provide a method for evaluating the heat resistance of corals, which solves the technical problem of species selection during the restoration of coral reefs after the existing artificial cultivation and domestication of corals
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Embodiment 1
[0032] Taking Porites lutea and Pavona decussata as examples, it involves the following three steps:
[0033] (1) Select corals under the ideal growth state, and set the water quality conditions of the control group culture tank (T: 26°C, KH: 7.0±0.3, pH: 8.2±0.1, Ca 2+ :400±15ppm, Mg 2+ :1400±20ppm, PO 4 3- 4 + 2 - 3 - ≈0ppm, light using 250W Metal halogen lamp+4T5HO, ODYSSEA), the collected coral individuals were adapted to the culture tank for 20 days, and the newly hatched brine shrimp (Artemia salina) was fed twice a week;
[0034] (2) Put the coral samples into the coral tank and adapt to recovery for 10-14 days.
[0035] (3) The stress temperature is set to 34°C, and the heat stress adopts a gradient temperature rise, which increases by 1°C in 12 hours, and acclimates for 3 days after rising to the target temperature;
[0036] (4) Using Diving-PAM underwater fluorometer (diving PAM underwater fluorometer) (WALZ, Germany) to measure and record the maximum light qu...
Embodiment 2
[0046] Taking Porites lutea and Pavona decussata as examples, it involves the following three steps:
[0047] (1) Select corals under the ideal growth state, and set the water quality conditions of the control group culture tank (T: 26°C, KH: 7.0±0.3, pH: 8.2±0.1, Ca 2+ :400±15ppm, Mg 2+ :1400±20ppm, PO 4 3- 3 + 2 - 3 - ≈0ppm, light using 250W Metal halogen lamp+4T5HO, ODYSSEA), the collected coral individuals were adapted to the culture tank for 20 days, and the newly hatched brine shrimp (Artemia salina) was fed twice a week;
[0048] (2) Set the temperature of the acclimation tank at 32°C for acclimatization, heat stress using a gradient temperature increase, 1°C in 12 hours, and acclimation for 3 days after reaching the target temperature;
[0049] (3) Transfer to 26 ℃ breeding tank, the water quality index is the same as that of the control group, and carry out secondary stress after 14 days of recovery;
[0050] (4) Set the secondary stress temperature to 34°C, he...
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Abstract
The invention discloses a method for assessing the heat resistance of a coral, and belongs to the field of ecological restoration of a marine environment. The method comprises the step that with phenotypic characteristics of the hermatypic coral, the symbiotic zooxanthellae density, the photosynthetic rate, superoxide dismutase (SOD), catalase (CAT), the aspartic acid protease (Caspase3) and the like as indexes, the heat resistance of the coral is assessed. The method has the advantages of multiple parameters, high precision, quantifiable comparison and the like, the method can be used for guiding coral transplanting and restoration, and a basis is provided for protection of a coral reef ecosystem. The method is easy and convenient to operate and easy to use, and the heat resistance of thecoral can be quickly assessed; by analyzing the phenotypic characteristics of the hermatypic coral, and the heat resistances of different kinds of corals can be compared so as to be used for seed selection in the coral breeding and coral transplanting and restoration processes; the heat resistances of the same kind of corals obtained before domestication and after domestication can be compared soas to used for assessing the domestication effect; moreover, the domestication effects of different kinds of corals can be compared so as to be used for seed selection during scientific research or coral transplanting and restoration.
Description
technical field [0001] The invention relates to the field of marine environment ecological restoration, in particular to a method for evaluating coral heat resistance. Background technique [0002] Coral reefs are the ecosystem with the highest species diversity and the richest resources in the world, known as the "tropical rainforest in the ocean". It is an important part of the global ecosystem, providing humans with resources such as food, coastal protection and medicine. However, in the past 50 years, under the influence of human activities and global warming, coral reefs around the world have been threatened to varying degrees and are undergoing rapid degradation. For example, the coastal reef coverage rate of Sanya Luhuitou in the South China Sea dropped from 80% to 90% in 1960 to 12% in 2009; the coral reef coverage rate of the Great Barrier Reef dropped from 28.0% in 1985 to 13.8% in 2012. Recently, The Nature Conservancy (TNC) pointed out that if the global temper...
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