Method for preparing angiosperm haploid from egg cell specific expression gene ECS and application of angiosperm haploid

Abstract

The invention discloses a method for preparing a plant haploid by regulating an egg cell specific expression gene and application of the method in preparation of angiosperm haploid. The aspartic protease ecs1 ecs2 double mutant specifically expressed by the egg cells can be used for producing haplobionts for breeding. The method overcomes the influence of deletion of haploid induced genes expressed by a traditional male parent on pollen and fertility, provides a new thought for the biological action of aspartic protease in the haploid production process, and can improve the breeding efficiency of crop haploids.

Description

[0001] Field of the invention: [0002] The invention belongs to the technical field of biological breeding, in particular, the invention relates to the application of the egg cell-specific expression gene ECS and its homologous gene in the preparation of angiosperm haploids. Background technique: [0003] Haploid plants have important advantages in genetic research and are also extremely important in crop breeding—they can be used to quickly generate homozygous diploids, thereby avoiding the manpower, material resources, and cost of producing near-isogenic lines caused by multiple generations of selfing. waste of time. Compared with traditional breeding methods such as gametophyte culture, distant hybridization, apomictic reproduction, etc., haploid breeding can significantly improve breeding efficiency and greatly shorten breeding time. However, directly cloning haploid-inducing genes in crops is a huge workload and technically difficult. In recent years, many researchers h...

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned genes can induce haploids, there are still problems to be solved urgently: first, mutations in the above-mentioned genes usually lead to abnormal pollen development, germination, or fertilization, which affects the rate of seed setting, thereby reducing the efficiency of haploid induction; Detecting male sterility in plants is an important method for quickly identifying haploids, but the male sterility phenotype caused by the above gene mutations greatly increases the difficulty of screening; third, it can only be obtained by crossing male-inducible lines with various crop lines For haploid plants, it is difficult to ensure the original excellent traits of crops or to avoid hybridization obstacles between certain varieties; fourth, the current haploid induction rate is low (Liu, C. et al. A 4-bp insertion atZmPLA1 encoding aputative phospholipase Agenerates haploid induction in maize. Mol. Plant, 2017, 10, 520–522; Gilles, L.M. et al. Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis inmaize. EMBO J., 2017, 36, 707–717. Yao, L. et al.OsMATL mutation induces haploidseed formation in indica rice.Nat.Plants,20184,530–533; Liu,C.et al.Extension of the in vivo haploid induction system from diploid maize to hexaploidwheat.Plant Biotechnol.J.,2020, 8, 316-318; Zhong, Y. et al. Mutation of ZmDMPenhances haploid induction in maize. Nat. Plants, 2019, 5, 575-580; Zhong, Y. et al. ADMP-triggered in vivo maternal haploid induction system in the dicotyledonous Arabidopsis. Nat. Plants, 2020, 6, 466-472; Li, Y., Lin, Z., Yue, Y. et al. Loss-of-function alleles of ZmPLD3 cause haploid induction in maize. Nat .Plants 2021, https: / / doi.org / 10.1038 / s41477-021-01037-2)

Images