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Quick measurement method for pyrazolone substances doped in medicaments and health-care food

A technology of health food and determination method, which is applied in the preparation of test samples, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., to achieve high accuracy, sensitive and rapid response, Ease of on-site operation

Active Publication Date: 2013-09-04
SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: (1) the chromatographic resolution is low, the composition of traditional Chinese medicine preparations is complex, and there are many interference factors, it is easy to misjudge some components as aminopyrine. The proximity of the biring will interfere with the detection of the target component
(2) The time for chromatographic development and drying is long, which cannot meet the requirements of rapid determination
(3) Thin-layer chromatography plates need to be stored dry, and the temperature and humidity of the environment have a great influence on the separation effect
(5) A fixed place is required, and it is not suitable for on-site testing with high mobility
The disadvantages are: (1) The instrument is expensive, the sample pretreatment time is long, the chromatographic column is easily polluted, and the analysis cost is high
(3) The instrument has high requirements on the use environment and needs to be placed fixedly, so it is not suitable for on-site testing with large fluidity
These two methods have high resolution and sensitivity, but they are not suitable for rapid screening and on-site detection with high mobility due to the expensive instruments required, complicated experimental operations, and high requirements for the environment in which the instruments are used.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Gastrodia elata capsules (capsules, marked dosage 2 capsules / time)

[0060] Take the content of 1 capsule, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put it in a 10ml sample tube, add 5ml of water, shake to make it fully dissolved, filter, collect the filtrate, and add to the filtrate Add 5ml of ethyl acetate to the mixture, shake for 2min, and let stand to obtain the ethyl acetate layer extract, transfer the extract and dry it with a hair dryer, add 4ml of water to the residue, shake to dissolve, and obtain the test solution. Adding 2% ferric ammonium sulfate solution to this solution, the solution produced a momentary purple color, and finally turned into a blue-purple color. Add 1 ml of 2% ferric ammonium sulfate solution to the extracted aqueous layer solution, and the solution does not change color.

[0061] Negative control sample test: take the content of 1 capsule of the same sample, put it in a 10ml sample tube, ...

Embodiment 2

[0063] Yaotongning Capsules (capsules, marked dosage 2 capsules / time)

[0064] Take the content of 1 capsule, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put it in a 10ml sample tube, add 5ml of water, shake to make it fully dissolved, filter, collect the filtrate, and add to the filtrate Add 5ml of ethyl acetate to the mixture, shake for 2min, and let stand to obtain the ethyl acetate layer extract, transfer the extract and dry it with a hair dryer, add 4ml of water to the residue, shake to dissolve, and obtain the test solution. Adding 1.8% ammonium ferric sulfate solution to this solution, the solution produced a momentary purple color, and finally turned into a blue-purple color. Add 1 ml of 1.8% ferric ammonium sulfate solution to the extracted aqueous layer solution, and the solution does not change color.

[0065] Negative control sample test: get 1 grain content of same sample, be placed in 10ml sample tube, add water and...

Embodiment 3

[0067] Rheumatoid Arthritis Tablets (yellow coated tablets, gray core, marked dosage 4 tablets / time)

[0068] Take 2 pieces of the sample, remove the coating, grind them in a mortar, add 0.01g of aminopyrine (equivalent to one-tenth of the clinical minimum effective dose), put them in a 10ml sample tube, add 5ml of water, shake to make it fully Dissolve, filter, collect the filtrate, add 5ml of ethyl acetate to the filtrate, shake for 2min, let it stand still to obtain the ethyl acetate layer extract, transfer the extract to dry with a hair dryer, add 4ml of water to the residue, shake to make Dissolved to obtain the test solution. Adding 1.5% ferric ammonium sulfate solution to this solution, the solution produced a momentary purple color and finally turned into a blue-purple color. Add 1ml of 1.5% ferric ammonium sulfate solution to the extracted aqueous layer solution, and the solution does not change color.

[0069] Negative control sample test: Take 2 tablets of the sam...

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Abstract

The invention discloses a quick measurement method for pyrazolone substances doped in medicaments and health-care food. The method comprises the following steps of: (1) extracting aminopyrine and similar components thereof in the medicaments and the health-care food to obtain extract; (2) further extracting the extract by using a solvent, and collecting extract and extracted solution serving as sample solution respectively, and (3) adding a developing agent into the sample solution respectively. The detection method is quick, simple and convenient, has strong specificity, high accuracy, sensitive response and wide application range, and is suitable for inspecting whether the medicaments and the health-care food are doped with the aminopyrine and the similar components thereof on site. A quick detection kit for the aminopyrine and the similar components thereof can meet the requirements of medicament development and health-care food supervision and inspection in vast rural areas and middle and small-sized cities with behindhand inspection technology.

Description

technical field [0001] The invention relates to a method for detecting pyrazolone chemical constituents aminopyrine and its analogues, in particular for detecting whether aminopyrine is added to anti-rheumatic traditional Chinese medicine preparations and health foods in medicines and health foods , Antipyrine and Analgin ingredients. Background technique [0002] Aminopyrine, the chemical constituent of pyrazolones, and its structural analogues have strong antipyretic and analgesic effects, which are slow and long-lasting, and their anti-inflammatory and anti-rheumatic effects are similar to aspirin. By inhibiting the synthesis and release of hypothalamic prostaglandins, it restores the reactivity of sensory neurons in the thermoregulatory center and plays an antipyretic effect; by inhibiting cyclooxygenase, it selectively inhibits the synthesis of prostaglandins in the hypothalamic thermoregulatory center, resulting in peripheral Dilate blood vessels and sweat to achieve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/28
Inventor 鲁艺钟敏赵承兵宋曼铜王珏肖丽和王铁杰
Owner SHENZHEN INST FOR DRUG CONTROL SHENZHEN TESTING CENT OF MEDICAL DEVICES
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