Laser scanning confocal microscope-based hair imaging method

Abstract

The invention provides a laser scanning confocal microscope-based hair imaging method, which is characterized by comprising the following steps of: marking hairs by using 0.5 percent acridine orange, and washing the hairs by using deionized water for 1 minute; and scanning a hair sample by using 488nm-wavelength laser of an argon ion laser of a laser scanning confocal microscope, and acquiring an image by using an XYZ scanning program. The method is easy to operate and the flaky structure of the hairs can be observed.

Description

technical field [0001] The invention provides a hair imaging method based on a laser scanning confocal microscope, which belongs to the technical field of microscope research. Background technique [0002] The laser scanning confocal microscope consists of a microscope optical system, a laser light source, a scanning device and a computer operating system. The entire set of instruments is controlled by a computer, and the operation switching between various components can be conveniently and flexibly carried out in the computer operating platform interface. The laser scanning confocal microscope uses laser light as the light source, and uses the illumination pinhole placed behind the light source and the detection pinhole placed in front of the detector to realize point illumination and point detection. The light emitted from the light source through the illumination pinhole is focused on the At a certain point on the focal plane of the sample, the emitted fluorescence at th...

AI Technical Summary

Problems solved by technology

[0004] Ordinary optical microscopes cannot be used to observe the scaly structure of hair. To observe the scaly structure of hair generally requires the use of scanning electron microscopy, but the preparation of samples for scanning electron microscopy is more complicated and the equipment is more expensive.

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