Laser scanning confocal microscope-based hair imaging method

A confocal microscope and laser scanning technology, applied in the field of microscope research, can solve the problems of complex preparation and expensive equipment, and achieve a wide range of applications

Inactive Publication Date: 2011-06-15
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Ordinary optical microscopes cannot be used to observe the scaly structure of hair. To observe the scaly structure of hair generally requires the us

Method used

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  • Laser scanning confocal microscope-based hair imaging method
  • Laser scanning confocal microscope-based hair imaging method

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Embodiment 1

[0012] Cut hair 1 cm, mark the hair with 0.5% acridine orange for 15 minutes, and rinse with deionized water for 1 minute; scan the hair sample with laser scanning confocal microscope argon ion laser with a wavelength of 488 nm, and use XYZ scanning program to collect images ; The wavelength of the collected signal is 520-600nm. This method is simple to operate and can observe the scaly structure of hair.

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Abstract

The invention provides a laser scanning confocal microscope-based hair imaging method, which is characterized by comprising the following steps of: marking hairs by using 0.5 percent acridine orange, and washing the hairs by using deionized water for 1 minute; and scanning a hair sample by using 488nm-wavelength laser of an argon ion laser of a laser scanning confocal microscope, and acquiring an image by using an XYZ scanning program. The method is easy to operate and the flaky structure of the hairs can be observed.

Description

technical field [0001] The invention provides a hair imaging method based on a laser scanning confocal microscope, which belongs to the technical field of microscope research. Background technique [0002] The laser scanning confocal microscope consists of a microscope optical system, a laser light source, a scanning device and a computer operating system. The entire set of instruments is controlled by a computer, and the operation switching between various components can be conveniently and flexibly carried out in the computer operating platform interface. The laser scanning confocal microscope uses laser light as the light source, and uses the illumination pinhole placed behind the light source and the detection pinhole placed in front of the detector to realize point illumination and point detection. The light emitted from the light source through the illumination pinhole is focused on the At a certain point on the focal plane of the sample, the emitted fluorescence at th...

Claims

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Application Information

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IPC IPC(8): G01N21/63
CPCG01N2021/8444G01N21/84
Inventor 刘东武陈志伟
Owner SHANDONG UNIV OF TECH
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