Method for extracting lipopolysaccharides from avian pasteurella multocida and preparing lipopolysaccharide vaccine

Abstract

The invention provides a method for extracting lipopolysaccharides from avian pasteurella multocida and preparing a lipopolysaccharide vaccine. The method comprises the following eight steps of: 1, preparing culture solution of avian pasteurella multocida; 2, collecting the avian pasteurella multocida from the culture solution of avian pasteurella multocida; 3, crushing the avian pasteurella multocida by using ultrasonic wave; 4, crudely extracting solution of lipopolysaccharides from the avian pasteurella multocida crushed by the ultrasonic wave; 5, extracting concentrated solution of lipopolysaccharides from the crudely extracted solution of lipopolysaccharides; 6, performing enzymolysis on deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in the concentrated solution of lipopolysaccharides by using DNA and RNA enzymes; 7, preparing the purified lipopolysaccharides; and 8, preparing the lipopolysaccharide vaccine of avian pasteurella multocida from the purified lipopolysaccharides. According to animal immunization experiments and animal virus attacking experiments, after the lipopolysaccharide vaccine of avian pasteurella multocida, prepared by the method, is used for immunizing chickens for three times, the immunized chickens can be effectively prevented from suffering from avian pasteurella multocida disease.

Description

technical field [0001] The invention belongs to the technical field of animal vaccine preparation, in particular to a method for extracting lipopolysaccharide from Pasteurella avium and preparing lipopolysaccharide vaccine. Background technique [0002] Pasteurellosis multocida is a contagious poultry infectious disease caused by Pasteurella multocida. It is distributed all over the world. It is also a common multiple infectious disease in my country. Almost all poultry can be infected with the disease. Infectious epidemics have seriously affected the healthy development of the poultry industry. [0003] At present, the prevention and treatment of poultry Pasteurella multocida mainly adopts drug therapy, such as antibiotics, but once the drug is stopped, it is easy to relapse, and long-term medication is easy to induce drug resistance of poultry Pasteurella multocida , It may also have side effects, such as a decrease in the egg production rate of laying hens. Therefore, in...

AI Technical Summary

Problems solved by technology

[0007] So far, there have been no relevant reports on the method of extracting lipopolysaccharide from Pasteurella avium multocida and making the lipopolysaccharide vaccine of Pasteurella multocida