Artificial nuclease and preparation method thereof

A technology of hydrolase and nucleic acid, applied in the field of biochemistry, to achieve efficient cutting effect
CN102120992AInactive Publication Date: 2011-07-13乔元彪 +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
乔元彪
Publication Date
2011-07-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses artificial nuclease and a preparation method thereof, belonging to the technical field of biochemistry and aiming at providing an efficient artificial nuclease and a preparation method thereof according to a restriction relation among the solubility, molecular structure, size and activity of the traditional nuclease. A water soluble functional metal compound is prepared from copper chloride, glycine and pi-electronic groups in a water phase through one-step chemical reaction, is of a plane or tetragonal pyramidal structure, and has strong insertion and hydrolysis capacity on nucleic acid, and stronger sequence selectivity and connection capacity; and by using a mixed solvent volatilizing method, the crystallizing efficiency of products is quickened, and the purity and the yield are improved. Copper at the center of the compound obtained from the invention is respectively of a three-dimensional tetragonal pyramidal structure and a plane quadrilateral structure, thus the artificial nuclease is endowed with tRNA targeting identification and biological connection genes, and has stronger binding constant to the nucleic acid and high hydrolysis rate.
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Description

technical field

[0001] The invention belongs to the technical field of biochemistry, and specifically relates to an artificial nucleolytic enzyme, and also relates to a preparation method of the artificial nucleic acid hydrolase. Background technique

[0002] Phosphodiester bonds in nucleic acids are fairly stable under physiological and non-enzymatic conditions. The widely used restriction endonuclease recognition sites are generally a few nucleotides, and they can only break nucleic acids at specific sites, so their use is limited. Chemically synthesized DNA / RNA cleavage compounds are internationally developed hydrolysis tools, which can effectively attack and hydrolyze phosphodiester bonds and generate 5'-, 3'-oligonucleoside phosphates. Artificial nucleases can effectively remove the contamination of pathogenic microorganisms such as bacteria and viruses; in addition, they have very important application value in modern molecular biology such as antisense nucleic acid, ...

Claims

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