Method for raising experimental population of Rhynchophorus ferrugineus

A red palm weevil, experimental technology, applied in the biological field, can solve the problems of short survival rate of developmental period, shortened generation cycle, etc., and achieve the effects of shortened generational cycle, short developmental period and wide source.

Inactive Publication Date: 2012-05-23
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of raising method of red palm weevil experimental population in view of the deficiencies in the prior art, adopts semi-artificial feed to finish the life history of red palm weevil indoors, can be under the situation of not harming egg and larva. A large number of intact eggs, accurate instar larvae and pupae are obtained, which have the characteristics of short development period and high survival rate, effectively shortening the generation cycle, in order to complete the biology, ecology and control technology of the red palm weevil. In-depth systematic research in the field provides a sufficient experimental population

Method used

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  • Method for raising experimental population of Rhynchophorus ferrugineus

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Experimental program
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Effect test

Embodiment 1

[0027] Select 50 red palm weevil pupae, and under 28 DEG C, 49 eclosion adults, put the red palm weevil adults into a glass bottle with 20% honey water according to the ratio of male to female ratio of 1:1, 1 pair of adult worms per bottle, 1-6 days later laying eggs, 430 eggs, 12 days egg period, routine disinfection of eggs (first soak in 10% formalin solution for 1 hour, then rinse with clean water and dry in the shade) , under the condition of 28 ℃, 412 hatched larvae were hatched, and the artificial feed was stirred and mixed according to the formula, and distributed into the feeding containers of the red palm weevil larvae, and the hatched larvae were inserted (one hatched larvae was inserted into each container) ), put them into a healthy insect breeding room, and changed the added feed at regular intervals depending on the feed situation. After 15 days, 321 larvae survived, and 268 larvae formed cocoons after 50 days. The cocoons were taken out and stored separately, an...

Embodiment 2

[0029] Select 40 red palm weevil pupae, and under 28 ℃ of conditions, 38 eclosion adults, red palm weevil adults are packed into a glass bottle equipped with 20% honey water according to the ratio of male to female ratio of 1:1. 1 pair of adults per bottle, 1-6 days after laying eggs, 450 eggs, egg stage 1-2 days, routine disinfection of eggs (first soak in 10% formalin solution for 1 hour, then rinse with clean water, dry in the shade Yes), under the condition of 28°C, 428 larvae were hatched, the artificial feed was stirred and mixed according to the formula, and packed into the feeding containers of red palm weevil larvae, and the hatched larvae were inserted into (each container was inserted into 1 out of larvae). 18 days later, 299 larvae survived, 185 cocoons formed after 48 days, the cocoons were taken out and stored separately, and 171 adults emerged after 32 days .

Embodiment 3

[0031] Select 60 pupae of red palm weevil, and under the condition of 28 ℃, 58 adults emerge, and the adult red palm weevil is packed into a glass bottle equipped with 20% honey water according to the ratio of male to female ratio of 1:1. 1 pair of adults per bottle (the cotton ball dipped in 20% honey water is suspended in a glass bottle for feeding and laying eggs), laying eggs after 1-6 days, 640 eggs, 1-2 days of egg stage, Eggs were routinely disinfected (soaked in 10% formalin solution for 1 hour, then rinsed with clean water and dried in the shade). At 28°C, 621 larvae were hatched, artificial feed was stirred and mixed according to the formula, and packed separately. In the feeding container of red palm weevil larvae, insert the hatched larvae (insert 1 hatched larvae in each container), put them into a healthy insect breeding room and raise them, and replace and add feed at regular intervals depending on the feed situation, 17 416 Tianhou larvae survived, 389 cocooned...

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Abstract

The invention relates to a method for raising an experimental population of Rhynchophorus ferrugineus. The method comprises the following steps of: performing eclosion on pupae of Rhynchophorus ferrugineus to be raised into worms, then putting each pair of adult worms which are taken as a group into a container with hydromel for mating and oviposition, routinely disinfecting eggs and then hatching to obtain larvae, inoculating the larvae into a Rhynchophorus ferrugineus larvae raising container loaded with a semi-artificial feed of the Rhynchophorus ferrugineus larvae, and putting into a healthy worm raising room for raising until the larvae are pupated. The method is simple, the life history of the Rhynchophorus ferrugineus is finished indoors by adopting the semi-artificial feed, and a large quantity of intact eggs, accurate larvae at various ages and pupae are obtained under the condition of not hurting the eggs and the larvae; and the method has the characteristics of short development duration, high survival rate and the like, effectively and greatly shortens the generation cycle, and provides adequate experiment populations for completing the deep and systemic research on the Rhynchophorus ferrugineus in the fields such as biology, ecology and control technologies and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for raising experimental populations of red palm weevils. Background technique [0002] The red palm weevil Rhynchophorus ferrugineus (Olivier) belongs to Coleoptera, Weevilidae, alias rusty palm weevil, coconut beetle, coconut beetle, Asian palm weevil, etc. It is a worldwide major pest that mainly damages palm plants , are distributed in Asia, Europe, and Africa, and are distributed in 12 provinces, municipalities and autonomous regions including Hainan, Guangxi, Guangdong, Zhejiang, Taiwan, Yunnan, and Tibet (Medog). [0003] The red palm weevil reproduces 2-3 generations in one year in my country, and the generations overlap. It can be harmful throughout the year. It is the most harmful to palm plants that are 3-15 years old. , cracks lay eggs, after the eggs hatch, the larvae bore and feed on plant tissues. There are no obvious symptoms at the beginning, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K1/18A23K10/18A23K10/30A23K10/37A23K20/105A23K20/147A23K20/158A23K20/163A23K20/174A23K50/90
CPCY02P60/87
Inventor 覃伟权阎伟刘丽黄山春李磊张晶
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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