Method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating

Abstract

The invention discloses a method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating, comprising the steps of selection of parents, parent fish cultivation and reproduction control, artificial insemination and incubation and cultivation of fish larvae and juvenile fish, wherein female verasper variegatus older than 3 years is selected to be used asa female parent, and male verasper moseri older than 2 years is selected to be used as a male parent; and the sexual maturity synchronization of the female parent fish and the male parent fish is promoted by means of temperature control, light control and nutrition enhancement. With respect to the artificial insemination, the insemination rate reaches 50%; the water temperature for incubation is (10+ / -0.5) DEG C, fish larvae can be incubated from inseminated eggs after 160 hours, and the incubation rate reaches more than 50%; and with respect to a feed series, L-type brachionus plicatilis andchlorella sp., artemia nauplii, and compound feed are respectively applied in different stages. The fish larvae (1-35 days in age) in an early stage are cultivated in a small water body (3m<3>) at the water temperature of (10+ / -0.5) DEG C; after 3 days, the temperature is raised by 0.5 DEG C every 2 days and is kept unchanged after reaching (15+ / -0.5) DEG C; and the fish larvae in a later stage are cultivated in a water body (30m<3>). After cultivating for 80 days, the survival rate of the fish larvae growing into the juvenile fish of 30mm in average is 20%. The method disclosed by the invention can be used for cultivating novel breeds and realizing scale breed reproduction.

Description

Technical field: [0001] The invention belongs to the seed breeding of seawater fishes in aquaculture, and specifically relates to a method for artificially hybridizing and cultivating new breeds and breeding seedlings by selecting female flounder and male flounder. Background technique: [0002] The round-spotted star flounder (Verasper variegates T.&S.) is mainly distributed in the Yellow Sea, Bohai Sea, East my country Sea, Japan, North Korea and other waters of China, and its output is not much. In the bay, there is no long-distance migration, and it is an excellent species for fishery resource proliferation and artificial breeding. Striped star flounder (Verasper moseri) is a cold-water benthic large-scale flounder, mainly distributed in the Pacific coast north of Ibaraki Prefecture in Japan and the Japan Sea coast north of Wakasa Bay. It was also distributed in the Yellow Sea and Bohai Sea in my country before, but it has not been seen for many years. See catch. Star fl...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is: by regulating the water temperature and light in the breeding environment, simulating the developmental law of the broodstock gonads in the natural environment, artificially stimulating and controlling the central nervous system and endocrine system of the fish, and promoting the growth of the female star flounder and striped flounder. The gonads of the male star flounder develop synchronously; through the method of artificial cross-breeding, the female star flounder × the male star flounder are crossed to breed new high-quality seedlings; at the same time, the technology of breeding new seedlings is explored

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