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PCR (polymerase chain reaction) quick salmonella detection primer based on gyrB gene

A technology for Salmonella and detection primers, which can be used in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., can solve problems such as inability to detect Salmonella, and achieve short detection cycles, high sensitivity, and reliable results. Effect

Inactive Publication Date: 2011-08-17
INST OF SOIL SCI CHINESE ACAD OF SCI
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Problems solved by technology

[0004] In the prior art, the use of PCR method has become one of the methods for rapid detection of Salmonella, but because the genes of the selected primers are usually functional genes, it is not possible to detect all Salmonella of the genus Salmonella, so there are certain limitations

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  • PCR (polymerase chain reaction) quick salmonella detection primer based on gyrB gene
  • PCR (polymerase chain reaction) quick salmonella detection primer based on gyrB gene
  • PCR (polymerase chain reaction) quick salmonella detection primer based on gyrB gene

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Embodiment

[0014] 1. Design of primers: Through comparative analysis of all known Salmonella genome sequences, select a segment with no secondary structure and a high degree of conservation (Salmonella gyrB Gene), design multiple pairs of primers, the length of the primers is generally about 20 bases, and there is no complementary sequence between the primers and within the primers. The optimal primer combination is as follows:

[0015] S-P-for: 5'-GGTGGTTTCCGTAAAAGTA-3'

[0016] S-P-rev: 5'-GAATCGCCTGGTTCTTGC-3'

[0017] 2. The primers are now used to detect 7 strains of common Salmonella, and at the same time, the accuracy and sensitivity of the rapid detection primers of the present invention are verified by using other common pathogenic strains in 6 strains of feces as controls.

[0018] Strains: Salmonella subgenus II Salmonella sub-genus II (CMCC50215), Salmonella subgenus III Salmonella sub-genus IIIb (CMCC50381), Salmonella Enteritidis Salmonella enteritidis (CMCC50335), ...

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Abstract

The invention discloses a PCR (polymerase chain reaction) quick salmonella detection primer based on a gyrB gene, comprising the following gene sequences: S-P-for: 5'-GGTGGTTTCCGTAAAAGTA-3'; and S-P-rev: 5'-GAATCGCCTGGTTCTTGC-3'. The primer disclosed by the invention has the advantages of short detection period, reliable result, high sensitivity and the like, is simple to operate, is favorable for the early clinical diagnosis of all common salmonella diseases in people and animals, and has an important meaning for detecting the pathogenicity salmonella in public health, animal husbandry and veterinary and port quarantine.

Description

[0001] technical field [0002] The invention belongs to the technical field of detection of pathogenic bacteria, in particular to a pair of gyrB Gene, PCR primers for rapid detection of Salmonella. Background technique [0003] Salmonella is one of the main pathogenic bacteria that cause food poisoning and foodborne diseases, and the incidence of diseases caused by Salmonella is increasing year by year. In 2008, an outbreak of Salmonella infection in St. Paul, USA, spread to 23 states, infected 228 people, and caused one death. my country's General Administration of Quality Supervision, Inspection and Quarantine clearly stipulates that Salmonella is a mandatory inspection item. Therefore, accurate and rapid detection of Salmonella in food and environment is of great significance for the prevention and control of Salmonella. [0004] In the prior art, the use of PCR method has become one of the methods for rapid detection of Salmonella, but because the genes of the sele...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/10C12N15/11
CPCY02A50/30
Inventor 叶旭红王一明林先贵
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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