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Preparation method of various small molecule activated peptides converted from special biological fungi for EFD (Engineered Fluid Dispensing)

A technology of small molecular peptides and special bacteria is applied in the field of preparation of various active small molecular peptides by bioconversion of EFD special bacteria, which can solve the problems of inability to achieve complete decomposition of soybean protein, complex process and high cost, and achieve pure smell and simple process. , good taste effect

Inactive Publication Date: 2011-08-24
朱立良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the technical problems of complex process, high cost, and inability to completely decompose soybean protein in the existing technology of extracting soybean protein peptides by protease hydrolysis, the present invention provides a special EFD bacteria for biological conversion of various active small molecular peptides The preparation method, this method is realized through the following steps:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of bioconversion of various active small molecule peptides by EFD special bacteria is realized through the following steps:

[0018] 1), according to the following weight percentages, take out 65% of soybean dehydrated protein powder with a purity of more than 50% to be decomposed, 5% of white sugar, 11% of EFD special bacteria, and the balance is purified water;

[0019] 2) Put the pure water and sugar into the container and stir. After the sugar dissolves, put in the EFD special bacteria to continue stirring. After stirring evenly, mix it with soybean dehydrated protein powder and put it in a thermostat at 38-40°C to seal and ferment. , the fermentation time is more than 72h;

[0020] 3) During the fermentation period, samples should be taken every 24 hours to test the increase of the bacterial population. During the fermentation process, the small peptide amino acids are metabolized and assimilated by microbial bacteria, and the soybean dehydra...

Embodiment 2

[0022] The preparation method of bioconversion of various active small molecule peptides by EFD special bacteria is realized through the following steps:

[0023] 1) Take out 70% of soybean dehydrated protein powder with a purity of more than 50% to be decomposed according to the following weight percentages, 4.5% of white sugar, 10.5% of EFD special bacteria, and the balance is purified water.

[0024] Other steps are with embodiment 1.

Embodiment 3

[0026] The preparation method of bioconversion of various active small molecule peptides by EFD special bacteria is realized through the following steps:

[0027] 1) Take out 75% of soybean dehydrated protein powder with a purity of more than 50% to be decomposed according to the following weight percentages, 4% of white sugar, 10% of EFD special bacteria, and the balance is purified water.

[0028] Other steps are with embodiment 1.

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Abstract

The invention discloses a preparation method of various small molecule activated peptides converted from special biological fungi for EFD (Engineered Fluid Dispensing), wherein the method provided by the invention is designed for solving the technical problems of complex process, high cost and incapability of completely decomposing soy protein when extracting soy protein by adopting the traditional proteinase hydrolization method. The method comprises the following steps: weighing 65-75 percent by weight of dewatered soy protein powder, 4-5 percent by weight of white sugar, 10-11 percent by weight of special fungi for EFD and the balance of purified water; placing the purified water and the white sugar into a container to stir; and adding the speical fungi for EFD to continue stirring, then mixing with the dewatered soy protein powder into a whole, and placing in a constant-temperature box at a temperature of 38-40 DEG C to seal and ferment for over 72 hours. The method provided by the invention has the beneficial effects that the process is simple and convenient, small peptide amino acid comprehensively decomposes soy protein into various activated peptides after being assimilated by the microbial fungi metabolism, the content of fungi in per gram is over 10*10<11>, and pure smell and good mouthfeeling are achieved; the decomposing process is finished at one step, and the original complex production process is replaced. The small molecule activated peptides can be widely applied to fields of biomedicine, foods, health protection, pathology research and the like and can also be used as additives of feeds in the animal husbandry.

Description

Technical field: [0001] The invention relates to a preparation method of various active small molecule peptides, in particular to a preparation method of biotransformation of various active small molecule peptides by EFD special bacteria. A variety of active small molecule peptides are widely used in the fields of biomedicine, food, health care and pathological research. It can also be used in animal husbandry as a feed additive. Background technique: [0002] At present, soybean protein peptides are generally extracted by protease hydrolysis, which is complex in preparation process, high in cost, and cannot completely decompose soybean protein. Invention content: [0003] In order to solve the technical problems of extracting soybean protein peptides by protease hydrolysis in the prior art, the process is complex, the cost is high, and the complete decomposition of soybean protein cannot be achieved, the present invention provides a kind of EFD special bacteria to biocon...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 于敏赵苓刘成立朱立良
Owner 朱立良
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