Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane

A nitrocellulose membrane and bioartificial technology, applied in the direction of suction equipment, etc., can solve the problems of difficulty in providing a three-dimensional hepatocyte culture microenvironment, lack of immune barrier and perfusion, uneven distribution of hepatocytes, etc., to avoid cell escape, Good cell compatibility, to achieve the effect of immune barrier

Inactive Publication Date: 2013-03-27
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the perfusion stent bioreactor has a good hepatocyte culture effect, but lacks immune barrier and uneven perfusion
Hollow fiber bioreactor is the most researched type of reactor, which has strong immune barrier and material transfer function, but the distribution of hepatocytes in this reactor is uneven, easy to deposit at the bottom, and it is difficult to provide three-dimensional hepatocyte culture Microenvironment

Method used

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  • Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane
  • Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane
  • Perfusion type bioartificial liver reactor based on double-layered nitrocellulose membrane

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Embodiment 1

[0022] Figure 1~3 The shown bioartificial liver reactor based on double-layer nitrocellulose membrane perfusion is an embodiment of the present invention, including a box body 1 and a cell scaffold 8 arranged in the inner cavity of the box body 1 . like figure 1 As shown, the cell support 8 adopts a double-layer nitrocellulose membrane, and the double-layer nitrocellulose membrane is arranged in the box body 1 in a wave-shaped folded shape. The left side and the right side of the box body 1 are defined as the two sides located in the direction of the long axis of the box body 1 . In the middle of the inner surface of the left side plate 2 of the casing 1 and the middle of the inner surface of the right side plate 3, grooves 9 equal in length to the two short sides of the double-layer nitrocellulose membrane are longitudinally arranged respectively, and the double-layer nitrocellulose membrane The two ends are fitted into the grooves 9 of the left side plate 2 and the right ...

Embodiment 2

[0025] like Figure 4 The shown bioartificial liver reactor based on double-layer nitrocellulose membrane perfusion is another embodiment of the present invention. The cell support 8 is folded in a W shape, and the top and bottom of the box 1 are aligned with the top of the bend. There is a space interval between them. The right side plate 3 of the box body 1 is a piston-type baffle plate that can perform linear reciprocating motion along the long axis direction in the box body. Adapt to different experimental needs.

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Abstract

The invention discloses a perfusion type bioartificial liver reactor based on a double-layered nitrocellulose membrane, which comprises a box body and a cell scaffold arranged in an intracavity of the box body, wherein a liquid inlet, a liquid outlet, an air inlet and an air outlet are arranged on the box body, and the cell scaffold is double-layered nitrocellulose membranes; the two long sides of the double-layered nitrocellulose membranes cling to the inner walls of the front side and the back side of the box body, and the two ends of the double-layered nitrocellulose membranes are fixed on the inner walls of the left side and the right side of the box body; and the double-layered nitrocellulose membranes divide the intracavity of the box body into upper and lower independent spaces, and a culture medium flows in the upper and lower independent spaces to exchange substances. An interval space between the two layers of the double-layered nitrocellulose membrane is used as a three-dimensional growth space of liver cells, and the cells grow in the interval space, so that substances are fully transmitted and the damage of shearing force to the cells when the liquid flows can be simultaneously avoided, and simultaneously the immune isolation effect can be achieved.

Description

technical field [0001] The invention relates to a bioartificial liver reactor, in particular to a bilayer nitrocellulose membrane perfusion bioartificial liver reactor. Background technique [0002] The bioreactor is the core device of the bioartificial liver system. It is an artificial organ that provides reliable over-supportive therapy for patients with liver failure. It has become an important means for patients with liver failure to wait for liver transplantation or provide a transition through their own liver regeneration. It is an essential supplement for liver transplantation and has broad application prospects in the treatment of liver failure. The artificial liver support system bioreactor must have two basic functions, one is to provide a good growth and metabolic microenvironment for liver cells, and the other is to provide an ideal environment for the interaction between blood or plasma and liver cells and material exchange in patients with liver failure. There...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61M1/22
Inventor 王亚东刘思德李爱民郑浩轩白杨刘址忠林建华
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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